4.2.1.69: cyanamide hydratase

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For detailed information about cyanamide hydratase, go to the full flat file.

Reaction

Synonyms

CAH, Cah protein, cyanamide-hydratase, hydratase, cyanamide, Urea hydro-lyase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.69 cyanamide hydratase

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Results	in table
22	AA Sequence
1	Application
1	CAS Registry Number
4	Cloned(Commentary)
5	Inhibitors
1	KM Value [mM]
1	Metals/Ions
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
7	Organism
2	Pathway
9	PDB ID
1	pH Optimum
1	pH Range
3	Purification (Commentary)
1	Reaction
1	Reaction Type
7	Reference
2	Specific Activity [micromol/min/mg]
6	Substrates and Products (Substrate)
1	Subunits
5	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]

Cloned

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Cloned on EC 4.2.1.69 - cyanamide hydratase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli, strains BL 21 (DE3) and JM109, pCAMBIA 1300 vector used for construction of gene cassette for genetic transformation in Nicotiana tabacum, cyanamide hydratase (cah) used as a marker gene

Nicotiana tabacum

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694750

the expression of the Cah enzyme in soybean leaves does not greatly affect the concentrations of any of the metabolites measured in unsprayed 1C and Jack plants grown under normal conditions or when placed in the dark for 24 h

Albifimbria verrucaria

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681141

transgenic soybean plants can be produced that express the fungal Cah gene and thus acquire resistance to toxic cyanamide. These plants are phenotypically normal and fertile indicating that expression of the Cah protein has no effect on soybean plant metabolism

Albifimbria verrucaria

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662714

wild-type cah and its mutants are cloned and overexpressed in pQE-60 Escherichia coli expression system. Western blot analysis confirms the production of 27700 Da cah enzyme by all the recombinants. Agrobacterium-mediated transformation is performed in Nicotiana tabaccum cv. Samsun plants by employing the leaf-disc method. The integration and expression of cah gene in transgenic plants are confirmed by polymerase chain reaction, Southern and Western blot analyses

Albifimbria verrucaria

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679392