4.2.1.66: cyanide hydratase

This is an abbreviated version!
For detailed information about cyanide hydratase, go to the full flat file.

Reaction

Synonyms

CHT, formamide dehydratase, formamide hydro-lyase, formamide hydro-lyase (cyanide-forming), formamide hydrolase, Formamide hydrolyase, hydratase, cyanide, More, NitAn1

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.66 cyanide hydratase

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Results	in table
4	Activating Compound
415	AA Sequence
10	Application
1	CAS Registry Number
14	Cloned(Commentary)
11	General Information
9	Inhibitors
5	KM Value [mM]
22	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
57	Organism
3	Pathway
9	pH Optimum
6	pH Range
1	pH Stability
1	Posttranslational Modification
21	Protein Variants
9	Purification (Commentary)
12	Reaction
1	Reaction Type
54	Reference
2	Source Tissue
8	Specific Activity [micromol/min/mg]
3	Storage Stability
76	Substrates and Products (Substrate)
14	Subunits
52	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]

Temperature Stability

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Temperature Stability on EC 4.2.1.66 - cyanide hydratase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

22 - 24

Microdochium sorghi

-

the activity is totally lost by incubation of the mycelia for 16 h at 22-24°C

704926

25

Stemphylium loti

-

50% loss of activity in 2 days

33944

27 - 50

3 entries

28 - 55

Aspergillus niger

-

the enzyme shows a half-life of 5.6 hat 28°C and is unstable at temperatures above 35°C. The enzyme is still active at 50°C with ca. 36% of its maximum activity, but nearly inactive at 55°C

730851

27 - 50

Aspergillus nidulans

-

incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, enzyme of Aspergillus nidulans quickly looses activity at the two highest temperatures, and 50% of activity is lost after 48 h at 37°C

690581

27 - 50

Fusarium graminearum

-

incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, CHT of Gibberella zeae shows an initial increase in activity at lower temperatures, subsequent stability is similar to that of Neurospora crassa, gradual decrease in activity at 50°C

690581

27 - 50

Microdochium sorghi

P32964

incubated at 27°C, 37°C, 43°C, and 50°C, activity measured at time points ranging between 0 and 48 h, enzyme quickly looses activity at the two highest temperatures, 50% of activity is lost after 48 h at 37°C

690581