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4.2.1.53: oleate hydratase

This is an abbreviated version!
For detailed information about oleate hydratase, go to the full flat file.

Word Map on EC 4.2.1.53

Reaction

(R)-10-hydroxystearate
=
oleate
+
H2O

Synonyms

Hydratase, oleate, OA hydratase, OHase, ohyA, oleate hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.53 oleate hydratase

Temperature Stability

Temperature Stability on EC 4.2.1.53 - oleate hydratase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 70
-
after 1 h incubation, the enzyme remains stable between 20 and 40°C (100% activity), at 45°C, 50°C, 55°C, 60°C, and 70°C it shows 80%, 70%, 45%, 20% and 0% residual activity, respectively
25 - 35
after 120 min, the enzyme activity is nearly unchanges at 25°C, but is reduced to only 25% at 35 °C
50
the immobilized enzyme (covalent immobilization of the enzyme using glutaraldehyde as cross-linking agent with chitosan-magnetic composite as carrier) preserves 40% of the initial activity, while the native enzyme is completely inactivated