4.2.1.47: GDP-mannose 4,6-dehydratase
This is an abbreviated version!
For detailed information about GDP-mannose 4,6-dehydratase, go to the full flat file.
Word Map on EC 4.2.1.47
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4.2.1.47
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alginate
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fucosylation
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gdp-l-fucose
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mucoid
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l-fucose
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gdp-4-keto-6-deoxy-d-mannose
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gdp-mannuronic
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gdp-d-rhamnose
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phosphomannomutase
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fucosyltransferase
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gdp-4-keto-6-deoxymannose
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d-rhamnose
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synthesis
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biotechnology
- 4.2.1.47
- alginate
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fucosylation
- gdp-l-fucose
- mucoid
- l-fucose
- gdp-4-keto-6-deoxy-d-mannose
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gdp-mannuronic
- gdp-d-rhamnose
- phosphomannomutase
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fucosyltransferase
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gdp-4-keto-6-deoxymannose
- d-rhamnose
- synthesis
- biotechnology
Reaction
Synonyms
ATCV-1GMD, bceN, Dm-gmd, GDP-alpha-D-mannose 4,6-dehydratase, GDP-D-mannose 4,6-dehydratase, GDP-D-mannose 4-oxido-6-reductase, GDP-D-mannose dehydratase, GDP-D-mannose-4,6-dehydratase, GDP-mannose 4, 6-dehydratase, GDP-mannose 4,6 dehydratase, GDP-mannose 4,6-dehydratase, GDP-mannose dehydratase, GDP-mannose dehydrogenase, GDP-mannose-4,6-dehydratase, GMD, GMDS, guanosine 5'-diphosphate-D-mannose 4,6-dehydratase, Guanosine 5'-diphosphate-D-mannose oxidoreductase, Guanosine diphosphomannose 4,6-dehydratase, Guanosine diphosphomannose oxidoreductase, L-GMD, M-GMD, MUR1, ORF13.7, PBCV-1 GMD, S-GMD
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Cloned
Cloned on EC 4.2.1.47 - GDP-mannose 4,6-dehydratase
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coexpression with GDP-4-keto-6-deioxymannose-3,5-epimerase-4-reductase, which is required for enzymatic activity after expresseion in Saccharomyces cerevisiae
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expressed in Escherichia coli as glutathione S-transferase fusion protein
expression in Escherichia coli
GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase, the two crucial enzymes for the de novo GDP-L-fucose biosynthesis, are overexpressed in recombinant Escherichia coli by constructing inducible overexpression vectors. Optimum expression conditions in recombinant Escherichia coli BL21(DE3) are 25°C and 0.1 mM isopropyl-beta-D-thioglucopyranoside. Maximum GDP-L-fucose concentration of 38.9 mg/l is obtained in a glucose-limited fed-batch cultivation, and it is enhanced further by coexpression of NADPH-regenerating glucose-6-phosphate dehydrogenase encoded by the zwf gene to achieve 55.2 mg/l GDP-L-fucose under the same cultivation condition
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GMD sequence determination and analysis, sequence comparison and phylogenetic tree, functional overexpression of soluble GMD in Escherichia coli strain BL21(DE3) from an IPTG-inducible expression vector, optimum expression conditions are 30°C and 0.1 mM IPTG
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into pET28a+ to construct pLW1534, expressed in Escherichia coli BL21 as His-tagged fusion protein
Paramecium bursaria Chlorella virus-1
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expressed in Escherichia coli as glutathione S-transferase fusion protein
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