4.2.1.42: galactarate dehydratase

This is an abbreviated version!
For detailed information about galactarate dehydratase, go to the full flat file.

Reaction

Synonyms

A9CG74, Dehydratase, galactarate, Galactarate dehydrase, galactarate dehydratase, galactarate dehydratase III, GalcD, GalrD, GalrD-III, L-talarate/galactarate dehydratase, m-galactarate dehydratase, STM3697

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.42 galactarate dehydratase

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Results	in table
3991	AA Sequence
4	Application
1	CAS Registry Number
6	Cloned(Commentary)
3	Crystallization (Commentary)
2	General Information
1	General Stability
6	Inhibitors
9	kcat/KM [mM/s]
2	Ki Value [mM]
10	KM Value [mM]
5	Metals/Ions
4	Natural Substrates/ Products (Substrates)
32	Organism
6	Pathway
6	PDB ID
3	pH Optimum
1	pH Range
11	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
11	Reference
1	Specific Activity [micromol/min/mg]
20	Substrates and Products (Substrate)
18	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
9	Turnover Number [1/s]

Engineering

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Engineering on EC 4.2.1.42 - galactarate dehydratase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

H45Q

Oceanobacillus iheyensis

-

has no detectable activity

702265

R162N

Oceanobacillus iheyensis

-

retains a small amount of activity

702265

Y164F

Oceanobacillus iheyensis

-

has no detectable activity

702265

Y90F

Oceanobacillus iheyensis

-

is catalytically impaired. Structure of the mutant in complex with Mg2+ and galactarate has a well-defined C-terminal segment through residue 387, well-ordered electron density for galactarate, and Mg2+ ions in both metal sites for both protomers comprising the asymmetric unit

702265

H328A

Salmonella enterica subsp. enterica serovar Typhimurium

Q8ZL58

inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate

678325

H328N

Salmonella enterica subsp. enterica serovar Typhimurium

Q8ZL58

inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate

678325

K197A

Salmonella enterica subsp. enterica serovar Typhimurium

Q8ZL58

inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate

678325

H328A

Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720

-

inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate

-

H328N

Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720

-

inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate

-

K197A

2 entries

K197A

Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720

-

inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate

-

K197A

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Q8ZL58

inactive mutant enzyme, the structure of the K197A mutant enzyme complexed with Mg2+ and L-glucarate is determined by molecular replacement using the SeMet-substituted STM3697 structure as the search model

678325