4.2.1.42: galactarate dehydratase

This is an abbreviated version!
For detailed information about galactarate dehydratase, go to the full flat file.

Reaction

Synonyms

A9CG74, Dehydratase, galactarate, Galactarate dehydrase, galactarate dehydratase, galactarate dehydratase III, GalcD, GalrD, GalrD-III, L-talarate/galactarate dehydratase, m-galactarate dehydratase, STM3697

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.42 galactarate dehydratase

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Results	in table
3991	AA Sequence
4	Application
1	CAS Registry Number
6	Cloned(Commentary)
3	Crystallization (Commentary)
2	General Information
1	General Stability
6	Inhibitors
9	kcat/KM [mM/s]
2	Ki Value [mM]
10	KM Value [mM]
5	Metals/Ions
4	Natural Substrates/ Products (Substrates)
32	Organism
6	Pathway
6	PDB ID
3	pH Optimum
1	pH Range
11	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
11	Reference
1	Specific Activity [micromol/min/mg]
20	Substrates and Products (Substrate)
18	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
9	Turnover Number [1/s]

Crystallization

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Crystallization on EC 4.2.1.42 - galactarate dehydratase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

sitting drop vapor diffusion method, using 2 M ammonium sulfate and 100 mM Bis-Tris-HCl (pH 5.5)

Agrobacterium tumefaciens

-

729259

structures of the wild type enzyme complexed with L-lyxarohydroxamate, and of the K197A mutant complexed with L-glucarate. Residue Lys 197 functions as the galactarate-specific base and His 328 functions as the L-talarate-specific base

Salmonella enterica subsp. enterica serovar Typhimurium

Q8ZL58

678325

three different crystal forms are grown by hanging drop vapor diffusion at room temperature: (1) selenomethionine (SeMet)-substituted wild type enzyme, (2) wild-type enzyme complexed with Mg2+ and L-lyxarohydroxamate, and (3) the K197A mutant enzyme complexed with Mg2+ and L-glucarate

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Q8ZL58

678325