4.2.1.42: galactarate dehydratase
This is an abbreviated version!
For detailed information about galactarate dehydratase, go to the full flat file.
Word Map on EC 4.2.1.42
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4.2.1.42
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enolase
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dehydration
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racemase
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mandelate
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departure
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agrobacterium
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alpha-proton
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aldolase
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dyad
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beta-strands
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glycerate
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d-galacturonate
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analysis
-
seventh
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epimerization
-
typhimurium
-
semialdehyde
- 4.2.1.42
- enolase
- dehydration
- racemase
- mandelate
-
departure
-
agrobacterium
-
alpha-proton
- aldolase
-
dyad
-
beta-strands
- glycerate
- d-galacturonate
- analysis
-
seventh
-
epimerization
- typhimurium
- semialdehyde
Reaction
Synonyms
A9CG74, Dehydratase, galactarate, Galactarate dehydrase, galactarate dehydratase, galactarate dehydratase III, GalcD, GalrD, GalrD-III, L-talarate/galactarate dehydratase, m-galactarate dehydratase, STM3697
ECTree
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Crystallization
Crystallization on EC 4.2.1.42 - galactarate dehydratase
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sitting drop vapor diffusion method, using 2 M ammonium sulfate and 100 mM Bis-Tris-HCl (pH 5.5)
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structures of the wild type enzyme complexed with L-lyxarohydroxamate, and of the K197A mutant complexed with L-glucarate. Residue Lys 197 functions as the galactarate-specific base and His 328 functions as the L-talarate-specific base
three different crystal forms are grown by hanging drop vapor diffusion at room temperature: (1) selenomethionine (SeMet)-substituted wild type enzyme, (2) wild-type enzyme complexed with Mg2+ and L-lyxarohydroxamate, and (3) the K197A mutant enzyme complexed with Mg2+ and L-glucarate