4.2.1.41: 5-dehydro-4-deoxyglucarate dehydratase
This is an abbreviated version!
For detailed information about 5-dehydro-4-deoxyglucarate dehydratase, go to the full flat file.
Word Map on EC 4.2.1.41
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4.2.1.41
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semialdehyde
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analysis
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d-galacturonic
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acidovorans
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tumefaciens
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d-glucuronic
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agrobacterium
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acinetobacter
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baylyi
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aldolase
- 4.2.1.41
- semialdehyde
- analysis
-
d-galacturonic
- acidovorans
- tumefaciens
-
d-glucuronic
-
agrobacterium
-
acinetobacter
- baylyi
- aldolase
Reaction
Synonyms
4-Deoxy-5-oxoglucarate hydro-lyase (decarboxylating), 5-dehydro-4-deoxyglucarate dehydratase, 5-keto-4-deoxy-D-glucarate dehydratase/decarboxylase, 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylase, 5-Keto-4-deoxy-glucarate dehydratase, D-4-Deoxy-5-ketoglucarate hydro-lyase, D-4-Deoxy-5-ketoglucarate hydro-lyase (decarboxylating), D-4-Deoxy-5-oxoglucarate hydro-lyase (decarboxylating), D-5-keto-4-deoxyglucarate dehydratase, Dehydratase, deoxyketoglucarate, Deoxyketoglucarate dehydratase, Deoxyoxoglucarate dehydratase, KDG dehydratase, KdgD, KDGDH, keto-deoxy-D-galactarate dehydratase
ECTree
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Purification
Purification on EC 4.2.1.41 - 5-dehydro-4-deoxyglucarate dehydratase
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expressed in Escherichia coli with a C-terminal Strep-tag to facilitate affinity purification of the protein
HisTrap column chromatography
using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column