4.2.1.36: homoaconitate hydratase
This is an abbreviated version!
For detailed information about homoaconitate hydratase, go to the full flat file.
Word Map on EC 4.2.1.36
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4.2.1.36
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alpha-aminoadipate
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homocitrate
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isopropylmalate
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auxotrophy
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homoisocitrate
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l-lysine
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lys4
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jannaschii
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saccharopine
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methanocaldococcus
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synthesis
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medicine
- 4.2.1.36
- alpha-aminoadipate
- homocitrate
- isopropylmalate
-
auxotrophy
- homoisocitrate
- l-lysine
- lys4
- jannaschii
- saccharopine
-
methanocaldococcus
- synthesis
- medicine
Reaction
Synonyms
2-hydroxy-3-carboxyadipate hydro-lyase, cis-Homoaconitase, HACN, HACNMj, Homoaconitase, Homoaconitate hydratase, Hydratase, homoaconitate, leuC, LYS4, lysF, MJ0499, MJ1271
ECTree
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Engineering
Engineering on EC 4.2.1.36 - homoaconitate hydratase
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R26K
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site-directed mutagenesis of small-subunit HACN protein MJ1271, the mutant variant forms a relatively efficient IPMI enzyme
R26V
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site-directed mutagenesis of small-subunit HACN protein MJ1271, the mutant variant forms a relatively efficient IPMI enzyme. The R26V variant shows detectable dehydratase activity with 3-isopropylmalate
R26V/T27Y
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site-directed mutagenesis of small-subunit HACN protein MJ1271, the mutant variant resembles the MJ1277 IPMI small subunit in its flexible loop sequence but demonstrates the broad substrate specificity of theR26V variant
T27A
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site-directed mutagenesis of small-subunit HACN protein MJ1271, the mutant variant has uniformly lower specificity constants for both IPMI and HACN substrates compared to the wild-type enzyme. In a holoenzyme complex, the T27A variant catalyzes the hydration of citraconate and maleate substrates with a 10fold higher KM than wild-type IPMIMj, and the KM values for cis-homoaconitate substrates increase 10-20fold relative to the wild-type HACNMj. The T27A variant has no detectable dehydratase activity with 3-isopropylmalate
additional information
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deletion of enzyme gene, results in almost avirulent mutant cells using a low dose intranasal mouse infection model
additional information
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site-directed mutagenesis of small-subunit HACN protein MJ1271 produces loop-region variant proteins that are reconstituted with wild-type MJ1003 large-subunit protein. The heteromers form promiscuous hydro-lyases with reduced activity but broader substrate specificity, overview
additional information
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small subunit of enzyme, expression in Thermus thermophilus. Gene product functions by forming a heterodimer with LysT subunit of Thermus thermophilus