4.2.1.33: 3-isopropylmalate dehydratase
This is an abbreviated version!
For detailed information about 3-isopropylmalate dehydratase, go to the full flat file.
Word Map on EC 4.2.1.33
Reaction
Synonyms
3-isopropylmalate dehydratase, 3-isopropylmalate isomerase, Alpha-IPM isomerase, alpha-isopropylmalate isomerase, beta-Isopropylmalate dehydratase, dehydratase,beta-isopropylmalate, IPM dehydratase, IPM isomerase, IPMI, IPMI SSU1, isopropylmalate dehydratase, isopropylmalate isomerase, LEU1S, leuC, LeuCD, LeuD, MJ0499, More, SOI10, Superoxide-inducible protein 10
ECTree
4.2.1.33 3-isopropylmalate dehydrataseAdvanced search results
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results (General Stability
General Stability on EC 4.2.1.33 - 3-isopropylmalate dehydratase
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GENERAL STABILITY 
ORGANISM
UNIPROT
LITERATURE
half-life 2-3 h in potassium phosphate buffer, ph 6.8., 0-5°C, beta-isopropylmalate at 1 mM increases half-life to 15 h
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loss of activity after repeated freezing and thawing
stabilized by high concentrations of glycerol and ammonium sulfate which also inhibit activity, half-life with 50% glycerol, 2.07 M ammonium sulfate, and 30% glycerol plus 830 mM ammonium sulfate: 50 h, 57 h, and 90 h, respectively, instead of 2-3 h without, stabilization probably due to changes in intramolecular interactions
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substrate stabilizes
unstable in phosphate buffer at pH 7.0, 10% loss of activity per h at 0°C
