4.2.1.30: glycerol dehydratase

This is an abbreviated version!
For detailed information about glycerol dehydratase, go to the full flat file.

Word Map on EC 4.2.1.30

4.2.1.30

pneumoniae

klebsiella

1,3-propanediol

diol

3-hydroxypropionaldehyde

3-hydroxypropionic

butyricum

adenosylcobalamin-dependent

adenosylcobalamin

aerobacter

synthesis

freundii

reactivase

pdors

2,3-butanediol

pasteurianum

1,2-ethanediol

poly3-hydroxypropionate

reuterin

adocbl

coenzyme-b12-dependent

analysis

biotechnology

Reaction

Synonyms

B12-dependent enzyme glycerol dehydratase, B12-dependent glycerol dehydratase, B12-independent GDH, B12-independent glycerol dehydratase, dehydratase, glycerol, DhaB, DhaB1, DhaBCE, DhaC, DhaE, GDH, GDHt, glycerol dehdydrogenase, glycerol dehydrase, glycerol dehydratase, glycerol hydro-lyase, glycerol hydrolyase, KpG, KpGDHt, vitamin B12-independent glycerol dehydratase

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.1 Hydro-lyases

4.2.1.30 glycerol dehydratase

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Results	in table
43	Activating Compound
411	AA Sequence
22	Application
1	CAS Registry Number
27	Cloned(Commentary)
63	Cofactor
5	Crystallization (Commentary)
13	General Information
4	General Stability
48	Inhibitors
9	kcat/KM [mM/s]
98	KM Value [mM]
32	Metals/Ions
49	Molecular Weight [Da]
78	Natural Substrates/ Products (Substrates)
176	Organism
2	Oxidation Stability
3	Pathway
12	PDB ID
15	pH Optimum
3	pH Range
4	pH Stability
38	Protein Variants
17	Purification (Commentary)
14	Reaction
1	Reaction Type
117	Reference
27	Specific Activity [micromol/min/mg]
3	Storage Stability
222	Substrates and Products (Substrate)
32	Subunits
66	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
2	Temperature Range [°C]
13	Temperature Stability [°C]
12	Turnover Number [1/s]

Purification

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Purification on EC 4.2.1.30 - glycerol dehydratase

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4 entries

apoenzyme, AB

Klebsiella pneumoniae

33823, 33824

by either ion exchange chromatography or on nickel chelate column

Klebsiella pneumoniae

702830

by Ni-nitrilotriacetate affinity chromatography followed by gel filtration

Klebsiella pneumoniae

704770

homogeneity, enzyme is inactive after purification, but can be reactivated with glycerol dehydratase-activating enzyme under anaerobic conditions

Clostridium butyricum

650250

homogeneity, recombinant enzyme, 2 different purification methods

Klebsiella pneumoniae

651133

immobilized metal ion affinity chromatography

Klebsiella pneumoniae

672728

inactive enzyme complex with hydroxocobalamin

Klebsiella pneumoniae

33819

Ni-nitrilotriacetate affinity chromatography followed by Sephacral S-300 gel filtration

2 entries

Ni-nitrilotriacetate agarose affinity column chromatography and Sephacryl S-300 gel filtration

Klebsiella pneumoniae

A8CIU9

729463

Ni-NTA agarose column chromatography and Sephacryl S-300 gel filtration

Citrobacter freundii

A8CH84; A8CH91; A8CH95

725951

recombinant His-tagged GDHt 2.11fold by nickel affinity chromatography and ultrafiltration

Klebsiella pneumoniae

A6NA24, A6NA25, Q59475

713813