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4.2.1.30: glycerol dehydratase

This is an abbreviated version!
For detailed information about glycerol dehydratase, go to the full flat file.

Word Map on EC 4.2.1.30

Reaction

glycerol
=
3-hydroxypropanal
 +
H2O

Synonyms

B12-dependent enzyme glycerol dehydratase, B12-dependent glycerol dehydratase, B12-independent GDH, B12-independent glycerol dehydratase, dehydratase, glycerol, DhaB, DhaB1, DhaBCE, DhaC, DhaE, GDH, GDHt, glycerol dehdydrogenase, glycerol dehydrase, glycerol dehydratase, glycerol hydro-lyase, glycerol hydrolyase, KpG, KpGDHt, vitamin B12-independent glycerol dehydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.30 glycerol dehydratase

Engineering

Engineering on EC 4.2.1.30 - glycerol dehydratase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R782K
-
forms a tight protein-protein complex with glycerol dehydratase-activating enzyme
I498A
-
optimal pH and optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
I498C
-
active, optimal pH as the wild-type, lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
I498D
-
active, lower optimal pH as the wild-type, optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
I498M
-
active, lower optimal pH and lower optimal temperature as the wild-type. Lower KM for glycerol as the wild-type
I498N
-
active, optimal pH as the wild-type, lower optimal temperature as the wild-type. Lower KM for glycerol as the wild-type
I498P
-
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
I498S
-
active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
I498T
-
active, lower optimal pH as the wild-type, optimal temperature as the wild-type. Lower KM for glycerol as the wild-type
I498V
-
alpha subunit has half-life of 11 h in contrast to 1 h for the wild-type. Optimal pH and optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
I498V/Q42L
-
alpha subunit of I498V and beta subunit of Q42L have half-life of 16 h in contrast to 1 h for the wild-type
I498W
-
active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
Q42A
-
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42C
-
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42D
-
most affected, alpha-helix around 152 positions is totally distorted. Higher optimal pH as the wild-type, lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42E
-
active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
Q42F
-
most active one, catalytic activity in beta-subunit is 8.3fold higher than the wild-type. Higher optimal pH and higher optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42G
-
active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
Q42H
-
active, optimal pH as the wild-type, higher optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
Q42I
-
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42K
-
active, higher optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42L
-
lower optimal pH and lower optimal temperature as the wild-type. Enzyme efficiency in beta-subunit for substrate glycerol is 336fold higher than that for 1,2-propanediol. Mutation at position of the beta-subunit contributes to the increase of the thermostability as well as the pH stability of the enzyme. Lower KM for glycerol as the wild-type
Q42M
-
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42N
-
most affected, alpha-helix around 152 positions is totally distorted. Higher optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
Q42P
-
active, optimal pH as the wild-type, lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42R
-
most affected, alpha-helix around 152 positions is totally distorted. Higher optimal pH and lower optimal temperature as the wild-type. Lower KM for glycerol as the wild-type
Q42S
-
enzyme efficiency in beta-subunit for substrate glycerol is 80fold higher than that for 1,2-propanediol. Higher optimal pH and higher optimal temperature as the wild-type. Lower KM for glycerol as the wild-type
Q42T
-
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42V
-
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
Q42W
-
active, lower optimal pH and lower optimal temperature as the wild-type. Lower KM for glycerol as the wild-type
Q42Y
-
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
T200S
4fold increase in catalytic efficiency of meso-2,3-butanediol dehydration
I498A
-
optimal pH and optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
-
I498C
-
active, optimal pH as the wild-type, lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
-
I498D
-
active, lower optimal pH as the wild-type, optimal temperature as the wild-type. Higher KM for glycerol as the wild-type
-
I498M
-
active, lower optimal pH and lower optimal temperature as the wild-type. Lower KM for glycerol as the wild-type
-
Q42E
-
active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type
-
additional information