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4.2.1.30: glycerol dehydratase

This is an abbreviated version!
For detailed information about glycerol dehydratase, go to the full flat file.

Word Map on EC 4.2.1.30

Reaction

glycerol
=
3-hydroxypropanal
 +
H2O

Synonyms

B12-dependent enzyme glycerol dehydratase, B12-dependent glycerol dehydratase, B12-independent GDH, B12-independent glycerol dehydratase, dehydratase, glycerol, DhaB, DhaB1, DhaBCE, DhaC, DhaE, GDH, GDHt, glycerol dehdydrogenase, glycerol dehydrase, glycerol dehydratase, glycerol hydro-lyase, glycerol hydrolyase, KpG, KpGDHt, vitamin B12-independent glycerol dehydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.30 glycerol dehydratase

Cloned

Cloned on EC 4.2.1.30 - glycerol dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
1,3-propanediol operon of dhaB1 (encodes the vitamin B12-independent glycerol dehydratase), dhaB2 (encodes the activating factor of DhaB1) tandemly arrayed with Escherichia coli yqhD gene (encodes 1,3-propanediol oxidoreductase) under the control of a constitutive, temperature-sensitive promoter, in the vector pBV220 for heterologous expression in Escherichia coli K-12 ER2925
a synthetic pathway 1,3-propanediol production pathway is introduced in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli
cloning of B12-independent glycerol dehydratase and its reactivase from Clostridium butyricum VPI1718, and expression in Escherichia coli BL21 (DE3). The activity of glycerol dehydratase activity is six times higher than in Clostridium butyricum
Q8GEZ8; Q8GEZ7
expressed in Escherichia coli JM109 cells
A8CH84; A8CH91; A8CH95
expressed in Escherichia coli XL10-Gold cells
expression in Escherichia coli
expression in Escherichia coli, which does not possess the 3-hydroxypropionic acid biosynthetic pathway
expression of the dhaB gene in Escherichia coli BL21(DE3)
genes dhaBCE, recombinant expression of His6-tagged GDHt in Escherichia coli strain Bl21(DE3), optimization of IPTG inducting process for recombinant GDHt to increase specific activity and ratio of soluble form using 0.8 mM IPTG at 20°C for 3 h, method development, overview
gldABC encoding for wild-type and mutant GDHt amplified, PCR fragment digested with NcoI and HindIII, inserted into pSE380 linearized with the same enzymes and transforming the competent cells of Escherichia coli JM109
-
overexpressed in Escherichia coli
PCR products of gdh genes for co-expressing alpha-, beta- and gamma subunits of wild-type GDH or GDHA/CB and GDHAB/C fusion constructs inserted into pET32a vector using NdeI/HindIII sites. To minimize the effect of steric hindrance, a linker peptide inserted into the fusion partners by SacI sites, resulting two plasmids (pET32aGDHA/CLB and pET32aGDHALB/C) for expressing fusion enzyme GDHA/CLB and GDHALB/C, expressed in Escherichia coli AD494(DE3)
-
pET-28a(+)-dhaBCEE expressed in Escherichia coli BL21 (DE3)
-
plasmid pCOLADuet-1::dhaB1B2::pduP::phaC1 expressed in Escherichia coli HMS174(DE3) cells
-
plasmid PKRX-T bearing the gene of the dhaBCE double digested with EcoR I and Sac I, resulting 2.7 kb fragment containing the intact dhaBCE genes inserted into pET-28a (+) vector linearized with the same enzymes. Transformation of the ligation mixture (pET-28 (+) dhaBCE) into Escherichia coli BL21 (DE3) for overexpression
the enzymes of pET-32a-gldABC (for cellular expression) and pET-22b-gldABC (for extracellular expression) are 6.8 and 3.2 U/mg, about six and three times that of the wild type enzyme, respectively. The empty expression plasmid pET-32a-gldAC containing no gldB is under construction to ligate a mutagenesis fragment of the gldB to construct a mutagenesis library
two classes of glycerol dehydratase in the organism, phylogenetic analysis, overview