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4.2.1.3: aconitate hydratase

This is an abbreviated version!
For detailed information about aconitate hydratase, go to the full flat file.

Word Map on EC 4.2.1.3

Reaction

citrate
=
cis-aconitate
 +
H2O

Synonyms

Acn, AcnA, AcnA3, AcnB, ACO, Aco1, Aco2, Aco3, ACO4, acon, aconitase, aconitase 2, aconitase A, aconitase B, aconitase/2-methylaconitate hydratase, Aconitate hydratase, AH, c-acon, c-aconitase, CAA, cis-aconitase, citB, citrate hydro-lyase, cytoplasmic aconitase, cytoplasmic aconitase/iron regulatory protein 1 homolog, EC 4.2.1.4, Ferritin repressor protein, hydratase, aconitate, IP210, IRE-BP, Iron regulatory protein, iron regulatory protein 1, iron regulatory-like protein, iron-regulatory protein 1, iron-responsive element binding protein, IRP, IRP-1, IRP1, mACON, Major iron-containing protein, MICP, More, PfIRPa, SPBP4H10.15

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.3 aconitate hydratase

Purification

Purification on EC 4.2.1.3 - aconitate hydratase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
AcnA and AcnB
-
both isoforms Aco1 and Aco4
-
enzyme form AcnA
-
isoenzymes Aco I and Aco II
Cucurbita sp.
-
native and SeMet [3Fe-4S] form of AcnB expressed in Escherichia coli
native IRP1 from liver
partial
partially, isolation of mitochondria
-
recombinant AcnB5-4 polypeptide
-
recombinant enzymes from Escherichia coli strain BL21(DE3) by gel filtration
recombinant His-tagged AcnA by metal affinity chromatography
recombinant His-tagged AcnB by metal affinity chromatography
recombinant His-tagged aconitase from Escherichia coli strain BL21(DE3) by nickel affinty chromatography and gel filtration, removal of the His tag by factor Xa and further purification, or purification of recombinant aconitase, expressed from plasmid pTYB2-acn in Escherichia coli, using chitin beads, overview
-
recombinant IRP1
-
using column chromatography
-
using Ni-NTA chromatography