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4.2.1.28: propanediol dehydratase

This is an abbreviated version!
For detailed information about propanediol dehydratase, go to the full flat file.

Word Map on EC 4.2.1.28

Reaction

Propane-1,2-diol
=
propanal
+
H2O

Synonyms

1,2-propanediol dehydratase, 1,2-propanediol hydro-lyase, adenosylcobalamin-dependent diol dehydratase, AdoCbl-dependent diol dehydratase, cobalamin-dependent diol dehydratase, coenzyme B12-dependent diol dehydrase, coenzyme B12-dependent diol dehydratase, coenzyme-B12-dependent diol dehydratase, DDH, dehydratase, diol, dehydratase, propanediol, diol dehydrase, diol dehydratase, diol dehydratase alpha subunit, dioldehydrase, dioldehydratase, DL-1,2-propanediol hydro-lyase, DL-1,2-propanediol hydrolyase, GldCDE, meso-2,3-butanediol dehydrase, PduCDE, PduCDEGH, Propanediol dehydrase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.28 propanediol dehydratase

Purification

Purification on EC 4.2.1.28 - propanediol dehydratase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and gel filtration
-
chromatography on DEAE-cellulose
-
DEAE-cellulose column chromatography
membrane-associated enzyme, higher yield than from cell-free extracts, 60000 subunit
-
native enzyme by ammonium sulfate fractionation and gel filtration
-
native enzyme by repeated immunoprecipitation on protein A-Sepharose beads, proteins fused to the N-terminus of PduD are encapsulated within the shell of the Pdu MCP
-