4.2.1.28: propanediol dehydratase

This is an abbreviated version!
For detailed information about propanediol dehydratase, go to the full flat file.

Reaction

Synonyms

1,2-propanediol dehydratase, 1,2-propanediol hydro-lyase, adenosylcobalamin-dependent diol dehydratase, AdoCbl-dependent diol dehydratase, cobalamin-dependent diol dehydratase, coenzyme B12-dependent diol dehydrase, coenzyme B12-dependent diol dehydratase, coenzyme-B12-dependent diol dehydratase, DDH, dehydratase, diol, dehydratase, propanediol, diol dehydrase, diol dehydratase, diol dehydratase alpha subunit, dioldehydrase, dioldehydratase, DL-1,2-propanediol hydro-lyase, DL-1,2-propanediol hydrolyase, GldCDE, meso-2,3-butanediol dehydrase, PduCDE, PduCDEGH, Propanediol dehydrase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.28 propanediol dehydratase

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Results	in table
12	Activating Compound
722	AA Sequence
2	Application
1	CAS Registry Number
31	Cloned(Commentary)
64	Cofactor
8	Crystallization (Commentary)
7	General Information
6	General Stability
95	Inhibitors
6	kcat/KM [mM/s]
17	Ki Value [mM]
71	KM Value [mM]
8	Localization
63	Metals/Ions
44	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
83	Organism
7	Oxidation Stability
3	Pathway
84	PDB ID
16	pH Optimum
3	pH Range
40	Protein Variants
17	Purification (Commentary)
15	Reaction
1	Reaction Type
81	Reference
2	Renatured (Commentary)
2	Source Tissue
13	Specific Activity [micromol/min/mg]
2	Storage Stability
125	Substrates and Products (Substrate)
27	Subunits
69	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
30	Turnover Number [1/s]

KM Value

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KM Value on EC 4.2.1.28 - propanediol dehydratase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.6

(R)-1,2-Propanediol

Klebsiella pneumoniae

-

-

5617

0.47 - 3.9

(R)-Propane-1,2-diol

4 entries

0.186

(S)-1,2-Propanediol

Klebsiella pneumoniae

-

-

5617

0.11 - 2.6

(S)-propane-1,2-diol

4 entries

2.5

1,2-Butanediol

Klebsiella oxytoca

-

pH 8.0, 37°C

698651

0.56 - 5.5

1,2-ethanediol

5 entries

0.04 - 10

1,2-propanediol

11 entries

0.64

2,3-Butanediol

Klebsiella pneumoniae

-

-

5616

0.00082 - 0.0027

adenosylcobinamide 3-benzimidazolylpropyl phosphate

2 entries

0.00099

adenosylcobinamide 3-imidazolylpropyl phosphate

Klebsiella oxytoca

-

-

651798

0.00029

adenosylcobinamide 3-pyridylpropyl phosphate

Klebsiella oxytoca

-

-

651798

0.26 - 0.9

Cobalamin

2 entries

0.00096

cobeta-adenosyl-coalpha-benzimidazolylcobamide

Klebsiella oxytoca

-

-

651798

0.00104

cobeta-adenosyl-coalpha-imidazolylcobamide

Klebsiella oxytoca

-

-

651798

0.0008 - 0.008

coenzyme B12

2 entries

10.5

ethane-1,2-diol

Lentilactobacillus diolivorans

-

at pH 8.6 and 37°C

729472

0.73 - 16

glycerol

5 entries

0.12 - 1.9

Propan-1,2-diol

5 entries

0.031 - 1.9

Propane-1,2-diol

9 entries

0.3

RS-1,2-propanediol

Klebsiella pneumoniae

-

-

5617

additional information

additional information

8 entries

-

0.47

(R)-Propane-1,2-diol

Klebsiella oxytoca

Q59470

wild type enzyme, at pH 8.0 and 37°C

729706

0.7

(R)-Propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant enzyme S301A, at pH 8.0 and 37°C

729706

2.1

(R)-Propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant enzyme Q336A, at pH 8.0 and 37°C

729706

3.9

(R)-Propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant enzyme S301A/Q336A, at pH 8.0 and 37°C

729706

0.11

(S)-propane-1,2-diol

Klebsiella oxytoca

Q59470

wild type enzyme, at pH 8.0 and 37°C

729706

0.26

(S)-propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant enzyme S301A, at pH 8.0 and 37°C

729706

0.66

(S)-propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant enzyme Q336A, at pH 8.0 and 37°C

729706

2.6

(S)-propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant enzyme S301A/Q336A, at pH 8.0 and 37°C

729706

0.56

1,2-ethanediol

Klebsiella pneumoniae

-

membrane-associated enzyme

5635

1.08

1,2-ethanediol

Klebsiella pneumoniae

-

both cytosolic and membrane associated enzymes

5616, 5629

3

1,2-ethanediol

Terrisporobacter glycolicus

-

-

5628

5.3

1,2-ethanediol

Levilactobacillus brevis

-

-

5626

5.5

1,2-ethanediol

Secundilactobacillus collinoides

-

-

651140

0.04

1,2-propanediol

Klebsiella pneumoniae

-

-

675575

0.04

1,2-propanediol

Klebsiella oxytoca

-

-

675575, 675716

0.08

1,2-propanediol

Klebsiella pneumoniae

-

membrane-associated enzyme

5635

0.08

1,2-propanediol

Klebsiella oxytoca

-

pH 8.0, 37°C

698651

0.08

1,2-propanediol

Klebsiella oxytoca

-

mutant S362A

675716

0.0814

1,2-propanediol

Klebsiella pneumoniae

-

-

5612

0.1

1,2-propanediol

Klebsiella oxytoca

-

mutant Q141A

675716

0.18

1,2-propanediol

Klebsiella pneumoniae

-

both cytosolic and membrane associated enzymes

5616, 5629

0.6

1,2-propanediol

Levilactobacillus brevis

-

-

5626

1.6

1,2-propanediol

Secundilactobacillus collinoides

-

-

651140

10

1,2-propanediol

Klebsiella oxytoca

-

mutant Q296A

675716

0.00082

adenosylcobinamide 3-benzimidazolylpropyl phosphate

Klebsiella oxytoca

-

-

651798

0.0027

adenosylcobinamide 3-benzimidazolylpropyl phosphate

Klebsiella oxytoca

-

-

651798

0.26

Cobalamin

Klebsiella oxytoca

-

pH 8.0, 37°C, mutant Halpha143Q

696252

0.9

Cobalamin

Klebsiella oxytoca

-

pH 8.0, 37°C, wild-type enzyme

696252

0.0008

coenzyme B12

Klebsiella oxytoca

-

-

651798

0.008

coenzyme B12

Secundilactobacillus collinoides

-

-

651140

0.73

glycerol

Klebsiella pneumoniae

-

membrane-associated enzyme

5635

1.54

glycerol

Klebsiella pneumoniae

-

cytosolic and membrane-associated enzymes

5616, 5629

4

glycerol

Levilactobacillus brevis

-

-

5626

8.3

glycerol

Secundilactobacillus collinoides

-

-

651140

16

glycerol

Klebsiella pneumoniae

-

-

5617

0.12

Propan-1,2-diol

Klebsiella oxytoca

-

pH 8.0, 37°C, mutant Kbeta135R

697886

0.15

Propan-1,2-diol

Klebsiella oxytoca

-

pH 8.0, 37°C, wild-type enzyme and mutant Salpha224A

697886

0.39

Propan-1,2-diol

Klebsiella oxytoca

-

pH 8.0, 37°C, mutant Kbeta135A and mutant Kbeta135Q

697886

0.4

Propan-1,2-diol

Klebsiella oxytoca

-

pH 8.0, 37°C, mutant Kbeta135E

697886

1.9

Propan-1,2-diol

Klebsiella oxytoca

-

pH 8.0, 37°C, mutant Salpha224N

697886

0.031

Propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant T172A, pH not specified in the publication, temperature not specified in the publication

746754

0.15

Propane-1,2-diol

Klebsiella oxytoca

Q59470

wild-type, pH not specified in the publication, temperature not specified in the publication

746754

0.15

Propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant S224A, pH not specified in the publication, temperature not specified in the publication

746754

0.16

Propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant T172S, pH not specified in the publication, temperature not specified in the publication

746754

0.22

Propane-1,2-diol

Klebsiella oxytoca

-

pH 8.0, 37°C, wild-type enzyme

696252

0.3

Propane-1,2-diol

Klebsiella oxytoca

-

pH 8.0, 37°C, mutant Halpha143Q

696252

0.51

Propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant S224C, pH not specified in the publication, temperature not specified in the publication

746754

1.3

Propane-1,2-diol

Lentilactobacillus diolivorans

-

at pH 8.6 and 37°C

729472

1.9

Propane-1,2-diol

Klebsiella oxytoca

Q59470

mutant S224N, pH not specified in the publication, temperature not specified in the publication

746754

additional information

additional information

Klebsiella pneumoniae

-

-

5614

-

additional information

additional information

Klebsiella pneumoniae

-

-

5617

-

additional information

additional information

Klebsiella pneumoniae

-

-

5623

-

additional information

additional information

Citrobacter freundii

-

-

5623

-

additional information

additional information

Propionibacterium freudenreichii

-

-

5623

-

additional information

additional information

Propionibacterium freudenreichii

-

-

5627

-

additional information

additional information

Klebsiella oxytoca

-

kinetic analysis of mutant enzymes, overview

696252

-

additional information

additional information

Klebsiella oxytoca

-

kinetic analysis with substrate analogues 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol

698651

-