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4.2.1.20: tryptophan synthase

This is an abbreviated version!
For detailed information about tryptophan synthase, go to the full flat file.

Word Map on EC 4.2.1.20

Reaction

1-C-(indol-3-yl)glycerol 3-phosphate
=
indole
 +
D-glyceraldehyde 3-phosphate

Synonyms

alpha2beta2 tryptophan synthase, alphaTS, AtTSB1, beta subunit of tryptophan synthase, indoleglycerol phosphate aldolase, It-TSA, L-serine hydro-lyase (adding indoleglycerol-phosphate), L-tryptophan synthetase, PtTSA, Rv1612, synthase, tryptophan, TrB, Trp synthase, Trp synthase beta, TrpA, trpB, TrpB1, TrpB2, TrpB2a, TrpB2i, TrpB2o, TRPS, tryptophan desmolase, tryptophan synthase, tryptophan synthase alpha subunit, tryptophan synthase alpha-subunit, tryptophan synthase alpha2beta2 complex, tryptophan synthase beta, tryptophan synthase beta 1, tryptophan synthase beta subunit, tryptophan synthetase, TS, TSA, TSase, TSB, TSB1, TSbeta

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.20 tryptophan synthase

Storage Stability

Storage Stability on EC 4.2.1.20 - tryptophan synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, beta subunit, 50 mM sodium bicine buffer, pH 7.8, 1 mM EDTA, 0.04 mM pyridoxal 5'-phoshate, 2 mM DTT
-
-20°C, beta2-subunit, potassium phosphate buffer pH 6.5-6.6, EDTA, 2-mercaptoethanol, pyridoxal 5'-phosphate
-
-20°C, frozen solution or suspension in 50% saturated ammonium sulfate, 40% residual activity after 3 years
-
-20°C, lyophilized, several months stable
-
-75°C, or liquid N2, alpha subunit
-
0°C, in cellulose triacetate, 6 months
-
4°C, 1 M potassium phosphate buffer, pH 7.6, 1 mM pyridoxal 5'-phosphate, 2 mM PMSF, 24 h, 7% loss of activity
-