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4.2.1.20: tryptophan synthase

This is an abbreviated version!
For detailed information about tryptophan synthase, go to the full flat file.

Word Map on EC 4.2.1.20

Reaction

1-C-(indol-3-yl)glycerol 3-phosphate
=
indole
 +
D-glyceraldehyde 3-phosphate

Synonyms

alpha2beta2 tryptophan synthase, alphaTS, AtTSB1, beta subunit of tryptophan synthase, indoleglycerol phosphate aldolase, It-TSA, L-serine hydro-lyase (adding indoleglycerol-phosphate), L-tryptophan synthetase, PtTSA, Rv1612, synthase, tryptophan, TrB, Trp synthase, Trp synthase beta, TrpA, trpB, TrpB1, TrpB2, TrpB2a, TrpB2i, TrpB2o, TRPS, tryptophan desmolase, tryptophan synthase, tryptophan synthase alpha subunit, tryptophan synthase alpha-subunit, tryptophan synthase alpha2beta2 complex, tryptophan synthase beta, tryptophan synthase beta 1, tryptophan synthase beta subunit, tryptophan synthetase, TS, TSA, TSase, TSB, TSB1, TSbeta

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.20 tryptophan synthase

Cloned

Cloned on EC 4.2.1.20 - tryptophan synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alpha-subunit
-
coexpression of trpA and trp, encoding the alpha- and beta-subunit of the enzyme in Escherichia coli strain CB149 lacking the trp operon
-
DNA and amino acid sequence determination of the alpha-subunit of the enzyme, overexpression of the alpha-subunit and the larger N-terminal part of the alpha-subunit, amino acid residues 1-188, in inclusion bodies
-
Escherichia coli pretransformed with pSTB7, a high copy number plasmid expressing tryptophan synthase from Salmonella enterica, is commercially available (ATCC 37845)
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 cells
-
expressed in Escherichia coli T7-express cells
expressed in Escherichia coli trpB point mutant (YS111) cells
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli strain BL21(DE3)
-
expression of the alpha-subunit in strain BL21(DE3) as soluble and insoluble protein
-
expression of the enzyme complex in Escherichia coli
-
expression of wild-type and mutant F139W, T24M/F139W, and T24L/F139W alpha-subunits as monomers, expression of mutant T24A/F139W, T24S/F139W and T24K/F139W alpha-subunits as soluble dimers in strain RB797
-
expression of wild-type and mutants enzymes in Escherichia coli
-
expression of wild-type enzyme complex and of the beta-subunit dimer in Escherichia coli CB149
-
expressionof mutant S178P in Escherichia coli
-
genes trpA and trpB2i, expression of wild-type alpha- and beta-subunits, sTrpA and sTrpB2i, and of TrpA mutants in Escherichia coli strain Bl21(DE3)
-
genes trpA encoding the alpha-subunit and trpB encoding the beta-subunit are coexpressed or expressed individually in Escherichia coli strain JM109
-
genes trpA, trpB1, and trpB2, expression in Escherichia coli
-
glutathione S-transferase fusion protein with alpha and beta subunits
-
independent expression of alpha- and beta-subunit encoded by genes Tk-trpA and Tk-trpB in Escherichia coli
-
into the pDrive Cloning vector and into the pCRII vector
isolation of alpha subunit mutants
-
over-expressed in Mycobacterium tuberculosis H37Rv as a His-tagged fusion protein
-
overexpression in Arabidopsis and Solanum lycopersicum confers tolerance to cadmium stress
overexpression of the alpha-subunit in Escherichia coli
overexpression of the alpha-subunit in strain RB797
-
tryptophan synthase alpha2beta2 complex from Salmonella typhimurium is prepared from Escherichia coli containing the plasmid pEBA-10
-
TSB phylogenetic analysis and tree, functional expression of isozyme AtTSBtype2 in Escherichia coli
TSB phylogenetic analysis and tree, functional expression of isozyme ZmTSBtype2 in Escherichia coli
-
wild-type and P28L/Y173F double mutant alpha-subunits are overexpressed in Escherichia coli
-