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4.2.1.165: chlorophyllide a 31-hydratase

This is an abbreviated version!
For detailed information about chlorophyllide a 31-hydratase, go to the full flat file.

Reaction

3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a
=
3-deacetyl-3-vinylbacteriochlorophyllide a
+
H2O

Synonyms

2-vinyl bacteriochlorophyllide hydratase, 3-vinyl bacteriochlorophyllide a hydratase, 3-vinyl hydratase, 3-vinyl-bacteriochlorophyll hydratase, 31-hydratase, bacterial 3-vinyl hydratase, bchF, BchF hydratase, Cabther_B0080, cfxBchF, ClimR0003, ClimR0008, ClimR0017, craBchF, CT1421

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.165 chlorophyllide a 31-hydratase

Cloned

Cloned on EC 4.2.1.165 - chlorophyllide a 31-hydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene bchF, phylogenetic analysis, recombinant expression in Escherichia coli strain Bl21(DE3), quantitative RT-PCR expression analysis
gene bchF, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene bchF, recombinant overexpression in Escherichia coli strain BL21(DE3)
gene bchF, the enzyme is encoded in an operon, bchF-bchC-bchX,with two other single-copy genes required for BChl a biosynthesis
single copy gene, genotyping of BchF in Proteobacteria, Acidobacteria, Chloroflexi, and Gemmatimonadetes, phylogenetic analysis