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4.2.1.159: dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose 2,3-dehydratase

This is an abbreviated version!
For detailed information about dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose 2,3-dehydratase, go to the full flat file.

Reaction

dTDP-2,6-dideoxy-D-glycero-hex-2-enos-4-ulose
=
dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose

Synonyms

D-olivose, D-oliose and D-mycarose 2,3-dehydratase, EryBV, evaA, jadO, megBVI, mtmV, oleV, spnO, TDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.159 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose 2,3-dehydratase

Engineering

Engineering on EC 4.2.1.159 - dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose 2,3-dehydratase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E139Q
complete loss of catalytic activity
E190A
complete loss of catalytic activity
E190Q
complete loss of catalytic activity
E231A/E233A
mutant constructed to reduce surface entropy. None of the resulting crystals demonstrates improved diffraction properties
E316A/K317A
mutant constructed to reduce surface entropy. None of the resulting crystals demonstrates improved diffraction properties
E405
complete loss of catalytic activity
E405A
complete loss of catalytic activity
K289A/Q290A/E293A
mutant constructed to reduce surface entropy. None of the resulting crystals demonstrates improved diffraction properties
R381A
mutant with improved crystallization parameters, kinetic parameters similar to wild-type
E139Q
-
complete loss of catalytic activity
-
E190A
-
complete loss of catalytic activity
-
E190Q
-
complete loss of catalytic activity
-
E405A
-
complete loss of catalytic activity
-
R381A
-
mutant with improved crystallization parameters, kinetic parameters similar to wild-type
-