4.2.1.148: 2-methylfumaryl-CoA hydratase
This is an abbreviated version!
For detailed information about 2-methylfumaryl-CoA hydratase, go to the full flat file.
Reaction
Synonyms
erythro-beta-methylmalonyl-CoA hydrolyase, Hah_1340, MCD, Mch, mesaconyl-C1-CoA hydratase, mesaconyl-CoA hydratase, mesaconyl-coenzyme A hydratase, More
ECTree
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Substrates Products
Substrates Products on EC 4.2.1.148 - 2-methylfumaryl-CoA hydratase
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REACTION DIAGRAM
(2R,3S)-2-methyl-3-hydroxysuccinyl-C4-CoA + H2O
3-methylfumaryl-CoA + H2O
i.e. (S)-citramalyl-CoA
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3-methylfumaryl-CoA + H2O
(S)-citramalyl-CoA
i.e. mesaconyl-C4-CoA
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mesaconyl-C4-CoA + H2O
(2R,3S)-2-methyl-3-hydroxysuccinyl-C4-CoA + H2O
EC 4.2.1.153
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2-methylfumaryl-CoA + H2O
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the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation
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(2R,3S)-2-methylmalyl-CoA
2-methylfumaryl-CoA + H2O
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(2R,3S)-2-methylmalyl-CoA i.e. L-erythro-beta-methylmalyl-CoA = (2R,3S)-2-methyl-3-carboxy-3-hydroxypropanoyl-CoA
2-methylfumaryl-CoA i.e. (E)-3-carboxy-2-methylprop-2-enoyl-CoA
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(2R,3S)-2-methylmalyl-CoA
2-methylfumaryl-CoA + H2O
the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation
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(2R,3S)-2-methylmalyl-CoA
2-methylfumaryl-CoA + H2O
(2R,3S)-2-methylmalyl-CoA i.e. L-erythro-beta-methylmalyl-CoA = (2R,3S)-2-methyl-3-carboxy-3-hydroxypropanoyl-CoA
2-methylfumaryl-CoA i.e. (E)-3-carboxy-2-methylprop-2-enoyl-CoA
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(2R,3S)-2-methylmalyl-CoA
2-methylfumaryl-CoA + H2O
the equilibrium of the reaction is on the side of (2R,3S)-2-methylmalyl-CoA
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(2R,3S)-2-methylmalyl-CoA
2-methylfumaryl-CoA + H2O
Haloarcula hispanica ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755
the equilibrium of the reaction is on the side of (2R,3S)-2-methylmalyl-CoA
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(2R,3S)-2-methylmalyl-CoA
2-methylfumaryl-CoA + H2O
the equilibrium of the reaction is on the side of (2R,3S)-2-methylmalyl-CoA
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(2R,3S)-2-methylmalyl-CoA
i.e. mesaconyl-CoA. The enzyme catalyzes a reaction in the methylaspartate cycle
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2-methylfumaryl-CoA + H2O
(2R,3S)-2-methylmalyl-CoA
i.e. mesaconyl-CoA, i.e. mesaconyl-C1-CoA. The equilibrium of the reaction is on the side of (2R,3S)-2-methylmalyl-CoA
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2-methylfumaryl-CoA + H2O
(2R,3S)-2-methylmalyl-CoA
Haloarcula hispanica ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755
i.e. mesaconyl-CoA. The enzyme catalyzes a reaction in the methylaspartate cycle
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2-methylfumaryl-CoA + H2O
(2R,3S)-2-methylmalyl-CoA
Haloarcula hispanica ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755
i.e. mesaconyl-CoA, i.e. mesaconyl-C1-CoA. The equilibrium of the reaction is on the side of (2R,3S)-2-methylmalyl-CoA
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2-methylfumaryl-CoA + H2O
(2R,3S)-2-methylmalyl-CoA
i.e. mesaconyl-CoA. The enzyme catalyzes a reaction in the methylaspartate cycle
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2-methylfumaryl-CoA + H2O
(2R,3S)-2-methylmalyl-CoA
i.e. mesaconyl-CoA, i.e. mesaconyl-C1-CoA. The equilibrium of the reaction is on the side of (2R,3S)-2-methylmalyl-CoA
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(2R,3S)-2-methyl-3-hydroxysuccinyl-C1-CoA + H2O
the physiological substrate is mesaconyl-C1-CoA
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mesaconyl-C1-CoA
(2R,3S)-2-methyl-3-hydroxysuccinyl-C1-CoA + H2O
hydration of mesaconyl-C1-CoA to (2R,3S)-2-methyl-3-hydroxysuccinyl-C1-CoA (erythro-beta-methylmalyl-CoA), EC 4.2.1.148. The activity for hydration of mesaconyl-C1-CoA is an order of magnitude higher for the reverse reaction of beta-methylmalyl-CoA dehydration
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mesaconyl-C1-CoA
(2R,3S)-2-methyl-3-hydroxysuccinyl-C1-CoA + H2O
Haloarcula hispanica ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755
the physiological substrate is mesaconyl-C1-CoA
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mesaconyl-C1-CoA
(2R,3S)-2-methyl-3-hydroxysuccinyl-C1-CoA + H2O
the physiological substrate is mesaconyl-C1-CoA
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recombinant mesaconyl-CoA hydratase has a 3.5fold bias for the physiological substrate, mesaconyl-C1-CoA, compared to mesaconyl-C4-CoA. The enzyme is also active with mesaconyl-C4-CoA, (S)-citramalyl-CoA and (S)-malyl-CoA, and virtually has no activity with other tested enoyl-CoA/3-hydroxyacyl-CoA compounds. The enzyme activity is an order of magnitude higher for mesaconyl-C1-CoA/beta-methylmalyl-CoA compared to other substrates
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additional information
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recombinant mesaconyl-CoA hydratase has a 3.5fold bias for the physiological substrate, mesaconyl-C1-CoA, compared to mesaconyl-C4-CoA. The enzyme is also active with mesaconyl-C4-CoA, (S)-citramalyl-CoA and (S)-malyl-CoA, and virtually has no activity with other tested enoyl-CoA/3-hydroxyacyl-CoA compounds. The enzyme activity is an order of magnitude higher for mesaconyl-C1-CoA/beta-methylmalyl-CoA compared to other substrates
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?
additional information
?
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Haloarcula hispanica ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755
recombinant mesaconyl-CoA hydratase has a 3.5fold bias for the physiological substrate, mesaconyl-C1-CoA, compared to mesaconyl-C4-CoA. The enzyme is also active with mesaconyl-C4-CoA, (S)-citramalyl-CoA and (S)-malyl-CoA, and virtually has no activity with other tested enoyl-CoA/3-hydroxyacyl-CoA compounds. The enzyme activity is an order of magnitude higher for mesaconyl-C1-CoA/beta-methylmalyl-CoA compared to other substrates
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?
additional information
?
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recombinant mesaconyl-CoA hydratase has a 3.5fold bias for the physiological substrate, mesaconyl-C1-CoA, compared to mesaconyl-C4-CoA. The enzyme is also active with mesaconyl-C4-CoA, (S)-citramalyl-CoA and (S)-malyl-CoA, and virtually has no activity with other tested enoyl-CoA/3-hydroxyacyl-CoA compounds. The enzyme activity is an order of magnitude higher for mesaconyl-C1-CoA/beta-methylmalyl-CoA compared to other substrates
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