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4.2.1.129: squalene-hopanol cyclase

This is an abbreviated version!
For detailed information about squalene-hopanol cyclase, go to the full flat file.

Reaction

hopan-22-ol
=
squalene
+
H2O

Synonyms

SHC, squalene-hopene cyclase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.129 squalene-hopanol cyclase

Purification

Purification on EC 4.2.1.129 - squalene-hopanol cyclase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native and/or recombinant enzyme, SHC in vivo is a membrane-associated protein and can be solubilized from cell extracts by nonionic detergents, such as Triton X-100 or octylthioglucopyranoside
the native enzyme can be solubilized from membranes by Triton X-100 and Tween 80 without loss of activity, but ionic detergents, such as 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), sodium-taurodeoxycolate, and different glucopyranosides, inhibit this enzyme more or less completely
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