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4.2.1.125: dammarenediol II synthase

This is an abbreviated version!
For detailed information about dammarenediol II synthase, go to the full flat file.

Word Map on EC 4.2.1.125

Reaction

dammarenediol II
=
(3S)-2,3-epoxy-2,3-dihydrosqualene
+
H2O

Synonyms

dammarenediol synthase, dammarenediol-II synthase, DDS, PgDDS, PgDDS1, PNA

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.125 dammarenediol II synthase

Cloned

Cloned on EC 4.2.1.125 - dammarenediol II synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, recombinant expression of functional His-tagged enzyme in Escherichia coli strains Origami B (DE3) amd Rosetta (DE3)
expression in an engineered strain of Saccharomyces cerevisiae strain 31147 from vector pAUR123, the enzyme is contained in microsomes, lipid particles, and total yeast homogenate
expression in Saccharomyces cerevisiae
-
functional expression in Sacchaormyces cerevisiae
gene DDS, functional recombinant overexpression in Oryza sativa cv. Shuhui 527 plants using the Agrbacterium tumefaciens transfection method, transfer-DNA (T-DNA) insertion sites in homozygous lines of the T2 generation are determined by using high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR), quantitative RT-PCR expression analysis
gene DDS, genotyping of cv. DAMAYA, identification of single nucleotide polymorphisms
gene DDS, recombinant expression in Pichia pastoris using the promoter of the AOX1 gene, coexpression with an ERG1 gene and an ERG7 gene
gene DDS, recombinant expression in Saccharomyces cerevisiae strain WTE (strain W303-1a integrated with pRS304-tHMG1 and pRS405-ERG20) from TEF1p-DS-ADH3t vector
gene PgDDS, functional expression in Nicotiana tabacum cv. Xanthi leaves using Agrobacterium strain GV3101, the heterologous expression confers resistance to Tobacco mosaic virus. Expression profile of PgDDS trangenic tobacco plants, overview. Production of dammarenediol-II in transgenic tobacco stimulates the expression of tobacco pathogenesis-related genes (PR1 and PR2) under both virus-untreated and -treated conditions
gene PNA, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of squalene synthase (UniProt ID P29704), squalene epoxidase (UniProt ID P32476), and NADPH-cytochrome P450 reductase (UniProt ID P16603) from Saccharomyces cerevisiae, and squalene epoxidase (UniProt ID Q603D5) from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana (UniProt ID Q9SB48)
gene PqDS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, ectopic heterologous co-expression with cytochrome P450 gene PqD12H in Saccharomyces cerevisiae
recombinant expression in Nicotiana tabacum cv. Xanthi leaves using transfection via Agrobacterium tumefaciens strain LBA4404, accumulation of dammarenediol-II in transgenic tobacco plants occurred in an organ-specific manner in descending order: roots, stems, leaves, flower buds, and transgenic cell suspension line 14 exhibits a high amount, overview