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4.2.1.109: methylthioribulose 1-phosphate dehydratase

This is an abbreviated version!
For detailed information about methylthioribulose 1-phosphate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.109

Reaction

5-(methylsulfanyl)-D-ribulose 1-phosphate
=
5-(methylsulfanyl)-2,3-dioxopentyl phosphate
+
H2O

Synonyms

1-PMT-ribulose dehydratase, 5'-methylthioribulose-1-phosphate dehydratase, 5-Methylthioribulose-1-phosphate, 5-methylthioribulose-1-phosphate dehydratase, 5’-methylthioribulose-1-phosphate dehydratase, Apaf-1 interacting protein, Apaf-1-interacting protein, APIP, Mde1p, MtnB, MtnBD, MTRu-1-P dehydratase, MTRu-1-P dehydratase/enolase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.109 methylthioribulose 1-phosphate dehydratase

Crystallization

Crystallization on EC 4.2.1.109 - methylthioribulose 1-phosphate dehydratase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged enzyme fragment of residues 20-242, hanging drop vapour diffusion method, 0.001 ml of protein solution, containing 20 mM Tris-HCl, 5% v/v glycerol, 0.1 mM TCEP, 100 mM sodium chloride, pH 8.0, is mixed with 0.001 ml of reservoir solution, containing 0.04 M citric acid, 0.06 M Bis-Tris propane, 5% v/v glycerol, 18-21% w/v PEG 3350, and equilibrated against 0.2 ml of reservoir solution, 22°C, 5 days to 2 weeks, X-ray diffraction structure determination and analysis at 2.40 A resolution