Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.2.1.108: ectoine synthase

This is an abbreviated version!
For detailed information about ectoine synthase, go to the full flat file.

Word Map on EC 4.2.1.108

Reaction

(2S)-4-acetamido-2-aminobutanoate
=
L-ectoine
 +
H2O

Synonyms

(Sa)Ect, EctC, ectoine synthase, L-ectoine synthase, SaEctC

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.108 ectoine synthase

Cloned

Cloned on EC 4.2.1.108 - ectoine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the expression vector pQE30 with a 6His-tag and expressed in Escherichia coli JM109 M15 cells
MH020164
expression of His6-tagged ectoine synthase in Escherichia coli strain BL21(DE3)
-
functional expression in transgenic Nicotiana tabaccum BY2 cells using the Agrobacterium tumefaciens infection system
-
gene ectC encoded in the ectABC gene cluster, functional expression in Escherichia coli
gene ectC located in the singel operon ectABCask, DNA sequence determination and analysis, genetic organization, expression in Escherichia coli strain X1-Blue
gene ectC, DNA and amino acid sequence determination and analysis, genetic mapping and organization, functional expression in Escherichia coli, the recombinant enzyme expression is osmoregulated in response to medium salinity in Escherichia coli
gene ectC, DNA and amino acid sequence determination and analysis, phylogenetic analysis and genetic organization
gene ectC, encoded in the ectABC gene cluster, sequence determination and analysis, phylogenetic analysis, DNA sequence comparison, expression in Escherichia coli
gene ectC, recombinant expression of a codon-optimized version of C-terminall Strep II-tagged enzyme in Escherichia coli strain BL21 under control of the tet promoter, which in turn is controlled by the TetR repressor, the genetic expression system can be induced by adding anhydrotetracycline
gene ectC, sequence comparisons, recombinant expression of a codon-optimized version of C-terminal Strep II-tagged enzyme in Escherichia coli strain BL21 from plasmid pNW12 under control of the tet promoter, which in turn is controlled by the TetR repressor, the genetic expression system can be induced by adding anhydrotetracycline, subcloning in Escherichia coli strain DH5alpha
strain TCJAR021, expression in Escherichia coli M15 (pREP4)
Halalkalibacterium halodurans
the ectABC gene cluster is expressed in Escherichia coli M15 (pREP4) by vector pQE30
Halalkalibacterium halodurans