4.2.1.104: cyanase
This is an abbreviated version!
For detailed information about cyanase, go to the full flat file.
Word Map on EC 4.2.1.104
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4.2.1.104
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bicarbonate
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bicarbonate-dependent
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anderson
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fuchs
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pseudoalcaligenes
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molecular biology
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nitrospira
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decameric
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environmental protection
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biotechnology
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drug development
- 4.2.1.104
- bicarbonate
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bicarbonate-dependent
-
anderson
-
fuchs
- pseudoalcaligenes
- molecular biology
- nitrospira
-
decameric
- environmental protection
- biotechnology
- drug development
Reaction
Synonyms
107368746, AtCYN, cyanase, cyanate aminohydrolase, cyanate C-N-lyase, cyanate hydratase, cyanate hydrolase, cyanate lyase, CYN, CynS, EC 3.5.5.3, EC 4.3.99.1, Hydrolase, cyanate, Ngar_c31480, NITMOv2_1274, OsCYN, slr0899, SpCynS, Spro_1533, tetur28g02430
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Substrates Products
Substrates Products on EC 4.2.1.104 - cyanase
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REACTION DIAGRAM
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enzyme could play a role in destroying exogenous cyanate originating from the dissociation of carbamoyl compounds such as urea, alternatively cyanate might constitute a convenient nitrogen source for bacteria able to synthesize cyanase in an inducible way
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Cyanate + bicarbonate
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cyanase-deficient strains have increased sensitivity to cyanate and are not able to use cyanate as nitrogen source
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Cyanate + bicarbonate
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Escherichia coli B / ATCC 11303
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breakdown of the inhibitory substance
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CO2 + carbamate
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Cyanate + bicarbonate
CO2 + carbamate
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initial product is carbamate or a related, unstable compound and/or carbamate precursor, which subsequently decomposes to ammonia and bicarbonate
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Cyanate + bicarbonate
CO2 + carbamate
Escherichia coli B / ATCC 11303
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initial product is carbamate or a related, unstable compound and/or carbamate precursor, which subsequently decomposes to ammonia and bicarbonate
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NH3 + 2 CO2
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utilization of CN and SCN as nitrogen sources, cyanase activity present if grown with SCN- and CN-
NH4 and CO2 are terminal nitrogen and carbon products of CN and SCN decomposition
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?
NCO- + HCO3- + 2 H+
NH3 + 2 CO2
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utilization of CN and SCN as nitrogen sources, cyanase activity present if grown with SCN- and CN-
NH4 and CO2 are terminal nitrogen and carbon products of CN and SCN decomposition
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?
NCO- + HCO3- + H+
NH3 + CO2
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utilization of CN- and SCN- as nitrogen sources, cyanase activity present if grown with SCN- and CN-
NH4 and CO2 are terminal nitrogen and carbon products of CN- and SCN- decomposition
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?
NCO- + HCO3- + H+
NH3 + CO2
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studies on cyanide and thiocyanate decomposition in Pseudomonas
after supply of CN- and SCN-, production of NH4+ observed at pH 8.8 in phosphate buffer, due to high rate of their utilization as N-source NH3 and CNO are absent from the culture liquids
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?
NCO- + HCO3- + H+
NH3 + CO2
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utilization of CN- and SCN- as nitrogen sources, cyanase activity present if grown with SCN- and CN-
NH4 and CO2 are terminal nitrogen and carbon products of CN- and SCN- decomposition
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?
NCO- + HCO3- + H+
NH3 + CO2
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studies on cyanide and thiocyanate decomposition in Pseudomonas
after supply of CN- and SCN-, production of NH4+ observed at pH 8.8 in phosphate buffer, due to high rate of their utilization as N-source NH3 and CNO are absent from the culture liquids
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?
NCO- + HCO3- + H+
NH3 + CO2
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studies on cyanide and thiocyanate decomposition in Pseudomonas
after supply of CN and SCN, production of NH4+ observed at pH 8.8 in phosphate buffer
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?
NCO- + HCO3- + H+
NH3 + CO2
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studies on cyanide and thiocyanate decomposition in Pseudomonas
after supply of CN and SCN, production of NH4+ observed at pH 8.8 in phosphate buffer
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?
NCO- + HCO3- + H+
NH3 + CO2
utilization of exogenous cyanate as a niche source of C and N in cyanobacteria
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NCO- + HCO3- + H+
NH3 + CO2
studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2
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?
NCO- + HCO3- + H+
NH3 + CO2
utilization of exogenous cyanate as a niche source of C and N in cyanobacteria
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?
NCO- + HCO3- + H+
NH3 + CO2
studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2
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-
?
NCO- + HCO3- + H+
NH3 + CO2
utilization of exogenous cyanate as a niche source of C and N in cyanobacteria
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-
?
NCO- + HCO3- + H+
NH3 + CO2
studies on decomposition of externally supplied cyanate in photoautotroph cyanobacteria
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?
NCO- + HCO3- + H+
NH3 + CO2
utilization of exogenous cyanate as a niche source of C and N in cyanobacteria
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?
NCO- + HCO3- + H+
NH3 + CO2
studies on decomposition of externally supplied cyanate in cyanobacteria
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?
NCO- + HCO3- + H+
NH3 + CO2
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cyanase activity in Thiohalophilus thiocyanoxidans, role in thiocyanate decomposition suggested
intermediates carbamate and CO2, spontaneous dissociation into NH3 and CO2
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?
NCO- + HCO3- + H+
NH3 + CO2
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thiocyanate metabolism of Thiohalophilus thiocyanoxidans
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NH4+ + 2 CO2
studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2
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?
NCO- + HCO3- + H+
NH4+ + 2 CO2
studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2
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-
?
NH4+ + CO2
utilization of exogenous cyanate as a niche source of C and N in cyanobacteria
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?
NCO- + HCO3- + H+
NH4+ + CO2
utilization of exogenous cyanate as a niche source of C and N in cyanobacteria
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activity is induced during growth with cyanide or cyanate, but not with ammonium or nitrate as the nitrogen source
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additional information
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no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation
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additional information
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cyanate is not a key intermediate in the degradation of cyanide, since the cynS mutant is still able to use cyanide as the sole nitrogen source
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additional information
?
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Pseudomonas oleovorans CECT 5344
no activity in cells grown with ammonium, nitrate, arginine or ornithine as N sources, cyanase of this strain has an assimilatory role but is not essential for cyanide assimilation
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?
additional information
?
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Pseudomonas oleovorans CECT 5344
cyanate is not a key intermediate in the degradation of cyanide, since the cynS mutant is still able to use cyanide as the sole nitrogen source
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?