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4.2.1.104: cyanase

This is an abbreviated version!
For detailed information about cyanase, go to the full flat file.

Word Map on EC 4.2.1.104

Reaction

Carbamate
+
H+
=
NH3
+
CO2

Synonyms

107368746, AtCYN, cyanase, cyanate aminohydrolase, cyanate C-N-lyase, cyanate hydratase, cyanate hydrolase, cyanate lyase, CYN, CynS, EC 3.5.5.3, EC 4.3.99.1, Hydrolase, cyanate, Ngar_c31480, NITMOv2_1274, OsCYN, slr0899, SpCynS, Spro_1533, tetur28g02430

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.104 cyanase

Engineering

Engineering on EC 4.2.1.104 - cyanase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E94L
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers
S117A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers
E94L
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers
-
S117A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers
-
E107A
site-directed mutagenesis, soluble, no activity
E107D
site-directed mutagenesis, soluble, no activity
R104A
site-directed mutagenesis, soluble, no activity
R104L
site-directed mutagenesis, soluble, no activity
S130A
site-directed mutagenesis, soluble, no activity
S130T
site-directed mutagenesis, soluble, no activity
additional information