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4.2.1.104: cyanase

This is an abbreviated version!
For detailed information about cyanase, go to the full flat file.

Word Map on EC 4.2.1.104

Reaction

Carbamate
+
H+
=
NH3
 +
CO2

Synonyms

107368746, AtCYN, cyanase, cyanate aminohydrolase, cyanate C-N-lyase, cyanate hydratase, cyanate hydrolase, cyanate lyase, CYN, CynS, EC 3.5.5.3, EC 4.3.99.1, Hydrolase, cyanate, Ngar_c31480, NITMOv2_1274, OsCYN, slr0899, SpCynS, Spro_1533, tetur28g02430

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.104 cyanase

Cloned

Cloned on EC 4.2.1.104 - cyanase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli, cyanase gene cynS not arranged in a three-gene cluster
expression in Escherichia coli DH5 alpha
expression in Escherichia coli strain M15
gene Atcyn, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 (DE3) as His-tagged soluble protein
gene cynS, cynS is presumably transcribed as part of the cynABDS operon, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene cynS, DNA and amino acid sequence determination and analysis
gene cynS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in Escherichia coli strain HMS174 as maltose-binding-protein fusion protein
gene cynS, DNA and amino acid sequence determination and analysis, tightly clustered with 2 genes located upstream, which encode proteins similar to the subunits of nitrate-nitrite transporter, complementation of deficient Escherichia coli strain
-
gene cynS, functional recombinant expression of C-terminally His-tagged enzyme in Arabidopsis thaliana plants ecotype Columbia under control of the CaMV 35S promoter via the Agrobacterium tumefaciens (GV3101)-mediated floral dip transformation method, ammonia release assay, pigment contents, and total carbohydrate measurements of transgenic plants, overview
gene cynS, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) or Trichoplusia ni High Five cells
gene cynS, tightly clustered with 4 putative molybdenum cofactor biosynthesis genes located downstream
-
gene Oscyn, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 (DE3) as His-tagged soluble protein
gene tetur28g02430, DNA and amino acid sequence determination and analysis, genomic and transcriptomic analysis, phylogenetic analysis, recombinant expression of MBP-tagged or N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene tetur28g02430, sequence comparisons and phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli BL21(DE3) pLys, the Tetranychus urticae cyanase remains functionally active after horizontal gene transfer