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4.2.1.1: carbonic anhydrase

This is an abbreviated version!
For detailed information about carbonic anhydrase, go to the full flat file.

Word Map on EC 4.2.1.1

Reaction

H2CO3
=
CO2
 +
H2O

Synonyms

(CA) XIV, AaCA1, alkalistable alpha-carbonic anhydrase, alpha carbonic anhydrase, alpha class carbonic anhydrase, alpha-CA, alpha-carbonic anhydrase, alpha-carbonic anhydrase isozyme II, alpha-class carbonic anhydrase, alpha-type CA, alpha-type carbonic anhydrase, alphaCA, alphaCA1, Am CA, AmCA, anhydrase, ASCA, AtCA1, AtCA2, AtCAL1, AtCAL2, B13-CA, BCA II, bCA IV, BCAII, BCAIIGln253Cys, beta carbonic anhydrase, beta-CA, beta-carbonic anhydrase, beta-class carbonic anhydrase, betaCA, betaCA1, BhCA, bovine carbonic anhydrase II, bsCA I, bsCA II, CA, CA 1, CA 3, CA I, CA II, CA III, CA IV, CA IX, CA VA, CA VB, CA VI, CA VII, CA VIII, CA XII, CA XIII, CA XIV, Ca XV, CA-I, CA-II, CA-III, CA-IX, CA-VA, CA-VB, CA-VI, CA-VII, CA-XII, CA-XIV, CA1, CA14x, CA2, CA2 homolog, CA2-like, CA3, CA4, CA5A, CA6, CA9, CAA, CAA1, CAA2, Cab, cab-type beta-class carbonic anhydrase, cadmium carbonic anhydrase, CAH3, CAH7, CAH8, CAI, CAII, CAIII, CAIV, CAIX, Cam, CamH, CaN, Can2, canA, canB, CaNce103, carbonate anhydrase, Carbonate dehydratase, carbonate dehydratase I, carbonate dehydratase III, Carbonate dehydratase IX, Carbonate dehydratase VA, Carbonate dehydratase VB, Carbonate dehydratase VI, Carbonate dehydratase VII, Carbonate dehydratase XII, Carbonate dehydratase XIV, carbonate hydro-lyase, carbonate hydrolase, carbonate hydrolyase, carbonic acid anhydrase, carbonic anhydrase, carbonic anhydrase 1, carbonic anhydrase 14, carbonic anhydrase 2, carbonic anhydrase 3, carbonic anhydrase 5A, carbonic anhydrase 6, carbonic anhydrase 9, carbonic anhydrase cambialistic enzyme, carbonic anhydrase I, carbonic anhydrase I (CA I) Michigan 1, carbonic anhydrase II, carbonic anhydrase III, carbonic anhydrase isozyme I, carbonic anhydrase isozyme II, carbonic anhydrase isozyme III, carbonic anhydrase isozyme IV, carbonic anhydrase isozyme IX, carbonic anhydrase IV, carbonic anhydrase IX, carbonic anhydrase type III, carbonic anhydrase V, carbonic anhydrase VA, carbonic anhydrase VI, carbonic anhydrase VII, carbonic anhydrase XII, carbonic anhydrase XIII, carbonic anhydrase XIV, carbonic anhydrase XV, carbonic anhydrase-I, carbonic anhydrase-II, carbonic anhydrase-related protein, carbonic anhydrase-related protein VIII, carbonic dehydratase, carboxyanhydrase, CARP, CAS1, CAS2, CasCAc, CasCAg, CAV, CAVA, CAXII, CcaA, CcmM, CDCA1, chloroplast carbonic anhydrase, clCA, CmCA, CO2 hydrase, CO2 hydratase, CPB, CpsCA, CpsCAgamma, CrCAH3, CsoSCA, cynT, CynT2, cytoplasmic carbonic anhydrase, Dca, dCA I, dCA II, dCAII, DDCA, dehydratase, carbonate, delta carbonic anhydrase, delta-carbonic anhydrase, delta-class CA, diatom carbonic anhydrase, Dsp-aCAopt sp., dual domain-carbonic anhydrase, dual-domain carbonic anhydrase, DVU_1777, E84A MTCA, eCA, ECCA, erythrocyte carbonic anhydrase, eta-CA, eta-carbonic anhydrase, external carbonic anhydrase, extracellular carbonic anhydrase, FbiCA 1, fCA, gamma carbonic anhydrase 1, gamma carbonic anhydrase 2, gamma carbonic anhydrase-like 1, gamma carbonic anhydrase-like 2, gamma-CA, gamma-CA1, gamma-CA2, gamma-CAL1, gamma-CAL2, gamma-carbonic anhydrase, gamma-class carbonic anhydrase, gammaCA, H216N ATCA, H64A HCA II, HC II, HCA, hCA I, HCA II, hCA III, hCA IV, hCA IX, hCA VA, hCA VB, hCA VI, hCA VII, hCA XII, hCA XIV, hCA XIV catalytic domain, HCA-I, HCA-II, hCAII, HcCA3, HICA, HP1186, hpalphaCA, hpbetaCA, human carbonic anhydrase, human carbonic anhydrase I, human carbonic anhydrase II, human carbonic anhydrase III, human carbonic anhydrase isoenzyme I, human carbonic anhydrase isoenzyme II, human carbonic anhydrase IX, human carbonic anhydrase XII, human carbonic anhydrase XIV, Ice-CA, icfA, isozyme CA II, isozyme CA IV, LdcCA, luminal carbonic anhydrase, lwCA, mCA V, mCA XIII, Membrane antigen MN, mesohalophilic carbonic anhydrase, MG-CA, MgaCA, More, mtcA1, mtcA2, MtCam, MTCY373.03, Nce103, NcoCA, NGCA, NstCcmM209, OEC33 protein, P54/58N, pentraxin-CA VI, pentraxin-carbonic anhydrase, PERMA_1443, PF3D7_1140000, PfCAdom, PgiCA, PgiCAb, PGJ_00014320, PhaCAgamma, PhCamH, photosystem II-associated carbonic anhydrase, Pl-can, plant-type (beta-class) carbonic anhydrase, PMCA, pMW1, poly-[Hb-SOD-CAT-CA], polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase, PtCA1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250, RT erythrocyte CA, Rv1248, Rv1284, Rv3273, Rv3588c, SABP3, Salivary carbonic anhydrase, SazCA, scCA, Secreted carbonic anhydrase, secretory carbonic anhydrase, secretory carbonic anhydrase VI, SspCA, STPCA, STPCA-2, SULAZ_0541, TacA, TCAb, TCAc, TcCA, Tcru, Tcr_1545, Theam_1576, tobacco salicylic acid-binding protein 3, Tumor antigen HOM-RCC-3.1.3, TWCA1, TweCA, VchCA

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.1 carbonic anhydrase

Purification

Purification on EC 4.2.1.1 - carbonic anhydrase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
agarose resin column chromatography
ammonium sulfate fractionation and 4-aminomethylbenzenesulfonamide agarose column chromatography
-
ammonium sulfate precipitation and Sephadex G-100 gel filtration
-
amylose resin chromatography
-
carbonic anhydrase 2
-
carbonic anhydrase inhibitor affinity chromatography
-
cytosolic isoenzyme
Elephas trogontherii
-
glutathione Sepharose 4B column chromatography and sulfonamide affinity chromatography
-
GSTrap resin chromatography and NHS-activated Sepharose 4 Fast Flow resin chromatography
-
His-Select column chromatography, gel filtration
Thermosynechococcus vestitus
-
His-tagged recombinant protein
-
improved method for the purification of the carbonic anhydrase isozymes by affinity chromatography
-
inner peripheral isoenzyme
Elephas trogontherii
-
integral isoenzyme
Elephas trogontherii
-
isozyme CA II, recombinant wild-type isozyme CA II and chimeric CA IX mimic mutant isozyme from Escherichia coli strain BL21(DE3) by p-aminomethylbenzenesulfonamide affinity chromatography
isozyme CA IX, recombinant wild-type isozyme from Escherichia coli strain BL21(DE3) by p-aminomethylbenzenesulfonamide affinity chromatography
mutant enzyme N67L is purified by affinity chromatography using 4-(aminomethyl)benzenesulfonamide coupled to agarose beads
mutant H64A
native enzyme 303.9fold from gills by L-tyrosine-sulfanilamide affinity chromatography
-
native enzyme 349fold by L-tyrosine-sulfanilamide affinity chromatography
-
native enzyme 35fold from spermatheca by L-tyrosine-sulfanylamide affinity chromatography, elution with 1 M NaCl/25 mM Na2HPO4, pH 6.3
-
native enzyme 539fold by one-step affinity chromatography on L-tyrosine sulphonamide
-
native enzyme 78.8fold from liver by single step tyrosine sulfanilamide affinity chromatography
-
native enzyme 91fold by L-tyrosine-sulfanilamide affinity chromatography, elution with 1 M NaCl/25 mM Na2HPO4, pH 6.3
-
native enzyme by anion exchange and hydrophobic interaction chromatography, followed by gel filtration
-
native enzyme from erythrocytes by gel filtration and electrofocusing
-
native enzynme from erythrocytes, 203fold by L-tyrosine sulfanilamide affinity chromatography
-
native enzynme from erythrocytes, 243fold by L-tyrosine sulfanilamide affinity chromatography, to homogeneity
-
native hCA I and hCA II from erythrocytes by aniline-sulfanylamide affinity chromatography
-
native isozyme hCA I from erythrocytes isolated from human blood by cellulose-benzyl-sulfanilamide affinity chromatography, elution with 1 M NaCl/25 mM Na2HPO4, pH 6.3
native isozyme hCA I from human blood erythrocytes by L-tyrosine-p-aminobenzene sulfonamide affinity chromatography
native isozyme hCA I from human blood erythrocytes by L-tyrosine-sulfanilamide affinity chromatography and elution with 1.0 M NaCl/25 mM Na2HPO4, pH 6.3
native isozyme hCA II from erythrocytes isolated from human blood by cellulose-benzyl-sulfanilamide affinity chromatography, elution with 0.1 M CH3COONa/0.5 M NaClO4, pH 5.6
native isozyme hCA II from human blood erythrocytes by L-tyrosine-sulfanilamide affinity chromatography and elution with 0.1 M NaCH3COO/0.5 M NaClO4, pH 5.6
native soluble enzyme from mantle by ammonium sulfate fractionation, anion exchange chromatography, and pAMBS affinity chromatography, to homogeneity
Ni-NTA column chromatography
-
Ni-NTA resin column chromatography
-
Ni-Sepharose column chromatography and Superdex S75 gel filtration
Ni2+ affinity column chromatography and Superdex 75 gel filtration
nickel affinity column chromatography
outer peripheral isoenzyme
Elephas trogontherii
-
protein A Sepharose CL-4B column chromatography
-
recombinant
recombinant Co-HICA from Escherichia coli by gel filtration
-
recombinant enzyme
recombinant enzyme CA VI-PTX from Spodoptera frugiperda Sf9 cells
recombinant enzyme from Escherichia coli anaerobically or aerobically, recombinant enzyme from Methanosarcina acetivorans anaerobically
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by 4-aminomethylbenzenesulfonamide agarose affinity chromatography and ultrafiltration
recombinant enzyme from Escherichia coli strain BL21(DE3) by p-(aminomethyl)-benzenesulfonamide affinity chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) by p-amino-methyl-benzenesulfonamide affinity chromatography and gel filtration, followed by ultrafiltration
recombinant enzymes
recombinant extracellular enzyme from Pichia pastoris strain GS115 culture supernatant by ultrafiltration, acetone precipitation, and pAMBS affinity chromatography
Halalkalibacterium halodurans
recombinant GST-tagged isozyme hCA IX from Escherichia coli strain BL21 Codon Plus by glutathione affinity chromatography and sulfonamide affinity chromatography
recombinant HCA II from Escherichia coli strain BL21
recombinant His-tagged bsCA II from Escherichia coli by cobalt affinityy chromatography
recombinant His-tagged enzyme
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatagraphy
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and gel filtration, followed by pAMBS affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) medium by nickel affinity chromatography
Halalkalibacterium halodurans
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/ArcticExpress DE3 cells by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by cobalt affinity chromatography and dialysis
recombinant His-tagged isozyme CA XIV from Escherichia coli strains to 96% purity by nickel affinity chromatography, dialysis, and 4-aminomethylbenzenesulfonamide affinity chromatography (elution by a linear gradient of 0.5 M sodium perchlorate using 0.1 M sodium acetate and 0-0.5 M sodium perchlorate, pH 5.6)
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged isozyme HCAII by nickel affinity chromatography, ultrafiltration, and gel filtration, to homogeneity
recombinant His-tagged PG domain of isozyme CA9 IX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by protease enzyme SenP2, and another step of nickel affinity chromatography to remove the tag
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and dialysis
recombinant is-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3)pLysS by nickel affinity chromatography, gel filtration, and tag cleavage by TEV protease, followed by dialysis, another step of nickel affinity chromatography, anion exchange chromatography, gel filtration, and ultrafiltration
recombinant isozymes CAH-3, CAH-4a and CAH-5 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant mutants V47A and G41A to homogeneity from Escherichia coli by ion exchange and hydrophobic interaction chromatography, and gel filtration
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography from the supernatant, and recombinant renatured His-tagged enzyme from inclusion bodies by nickel affinity chromatography, both enzyme forms are active
recombinant wild-type and mutant enzymes from Escherichia coli
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by affinity chromatography on a 4-(aminomethyl)-benzene-sulfonamide resin
-
Sephadex G-75 gel filtration, DEAE cellulose column chromatography, and Sepharose-4B-L tyrosine sulfanilamide column chromatography
-
Sepharose 4B-L-tyrosine sulfanilamide column chromatography
-
Sepharose 4B-L-tyrosine-sulfanilamide affinity chromatography
-
Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography
-
Sepharose-4B-L-tyrosine-sulfanilamide affinity column chromatography
-
Sepharose-6B-aniline-sulfanilamide affinity column chromatography
-
Sepharose-IDA-Ni+2 column chromatography, S-Sepharose column chromatography, gel filtration
Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, Bio-Scale CHT-20-I column chromatography, and Superdex 200pg gel filtration
Superdex 200 gel filtration and Superdex 75 gel filtration
Talon Co2+-affinity resin column chromatography, gel filtration
the enzyme from a commercial preparation is further purified by gel filtration and ultrafiltration
the recombinant enzyme is purified anaerobically
using Ni-NTA chromatography
-