beta class isozymes bsCA I and bsCA II, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged bsCA II in Escherichia coli
BhCA, recombinant constitutive extracellular expression of the enzyme in Pichia pastoris strain GS115 under methanol inducible (AOX1) as well as constitutive (GAP) promoters, the recombinant enzyme is secreted. The double transformant of the strain GS115 harboring BhCA under both AOX1 and GAP promoters possessed two copies of the gene
cloning of the full domain of carbonic anhydrase from Plasmodium falciparum, which incorporates 358 amino acid residues, from 181 to 538, in the sequence of this 600 amino acid long protein, called PfCAdom
DNA and amino acid sequence determination and analysis of PgiCAb, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli
expressed in Escherichia coli and in a baculovirus-Sf9 insect cell expression system, the recombinant proteins consist of either the CA IX catalytic domain only or the extracellular domain, which includes both the proteoglycan and catalytic domains, lacking the small transmembrane and intracytoplasmic regions of CA IX
gene Ca, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, comparative real-time quantitative PCR, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene CA14, recombinant expression in Escherichia coli: effect of different promoters, Escherichia coli strains (Rosetta 2(DE3), Rosetta-gami 2 (DE3), Origami B (DE3), and BL21 trxB (DE3)), and the length of recombinant CA XIV protein construct are analyzed for the production CA XIV in large scale by using affinity purification, method evaluation and optimization, overview. The most suitable Escherichia coli strain is Origami B (DE3)
gene CA2, cloned from DNA of outer and inner mantle, DNA and amino acid sequence determination and analysis, no nucleotide polymorphism is observed between the CA2-like cDNA sequences obtained from these two tissues, phylogenetic analysis, quantitative real-time PCR expression analysis of CA2-like
gene CA2, recombinant expression in Escherichia coli strain BL21(DE3), optimization of expression media and cell culture conditions to produce high levels of 3 different deuterated human carbonic anhydrases (hCAs). The labeled hCAs are then characterized and tested for deuterium incorporation by mass spectrometry, temperature stability, and propensity to crystallize, overview
gene CA5A, localized to chromosome 16q24.3, an unprocessed pseudogene is assigned to 16p12, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3) from plasmid pENTR223 as soluble enzyme and in inclusion bodies
gene ca6, isolation and sequencing of Danio rerio CA VI cDNA, complete with the sequence coding for the PTX domain, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of the recombinant CA VI-PTX protein in Spodoptera frugiperda Sf9 cells using the baculovirus expression system, quantitative real-time PCR
gene CA9, recombinant expression in Escherichia coli strain BL21(DE3), optimization of expression media and cell culture conditions to produce high levels of 3 different deuterated human carbonic anhydrases (hCAs). The labeled hCAs are then characterized and tested for deuterium incorporation by mass spectrometry, temperature stability, and propensity to crystallize, overview. Deuteration highly reduces the enzyme yield
genes cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6, real-time RT-PCR expression analysis, overview. Gene cah-3 is able to complement the function of Saccharomyces cerevisiae CA, NCE103, in vivo, expression in Saccharomyces cerevisiae wild-type strain CEN.PK2 and in mutant strain CEN.HE28-1A. Recombinant expression of His-tagged CAH-3, CAH-4a and CAH-5 in Escherichia coli strain BL21(DE3), but in vitro activity is only detectable for CAH-4a
isozyme carbonic anhydrase 6, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR and quantitative/semiquantitative RT-PCR expression analysis
isozyme LjCAA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis by quantitative real-time RT-PCR, and spatial expression profiling, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)
isozyme LjCAA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis by quantitative real-time RT-PCR, and spatial expression profiling, recombinant expression of His-tagged isozymes in Escherichia coli strain BL21(DE3)
overexpressed in transformed Escherichia coli cultures, expression in yeast, complements the growth defect in a yeast beta-CA knockout in Saccharomyces cerevisiae DELTA NCE103
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) achieving a 31.4fold enhancement in CA production for the wild-type, subcloning in Escherichia coli strain DH5alpha. About 50% of wild-type enzyme produced is secreted when recombinant Escherichia coli harboring BhCA-pET22b is cultivated in a medium with EDTA and lysozyme because of the efficient pelB leader sequence, cultvation parameters are optimized for rBhCA secretion
4.2.1.1 carbonic anhydrase
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