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4.1.99.5: aldehyde oxygenase (deformylating)

This is an abbreviated version!
For detailed information about aldehyde oxygenase (deformylating), go to the full flat file.

Word Map on EC 4.1.99.5

Reaction

octadecanal
+
O2
 + 2 NADPH + 2 H+ =
heptadecane
 +
formate
 +
H2O
 + 2 NADP+

Synonyms

ADO, aldehyde decarbonylase, aldehyde deformylase, aldehyde deformylating oxygenase, aldehyde-deformylating oxygenase, CAD, cADO, cADO-1593, cyanobacterial ADO, cyanobacterial aldehyde decarbonylase, cyanobacterial aldehyde deformylating oxygenase, cyanobacterial aldehyde-deformylating oxygenase, decarbonylase, erial aldehyde-deformylating oxygenase, LiADO, More, Npun_R1711, OsADO, PMT1231, PMT_1231, RS9917_09941, SeADO, sll0208, Synpcc7942_1593

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.5 aldehyde oxygenase (deformylating)

Cloned

Cloned on EC 4.1.99.5 - aldehyde oxygenase (deformylating)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity-tagged version expressed in Escherichia coli
-
cloning of gene Synpcc7942_159 from strain PCC7942, overexpression of N-terminally His-tagged protein in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli
-
expression of N-terminally His-tagged enzyme in Escherichia coli
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Npun_R1711, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PMT1231, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain BW25113(DE3) DELTA13
gene PMT_1231, recombinant expression in Escherichia coli
gene Synpcc7942_1593, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene Synpcc7942_1593, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
His-tagged version expressed in Escherichia coli
-
overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
recombinant expression in Escherichia coli
-
recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Prochlorococcus marinus), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase, ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
recombinant expression of mutants V41Y and A134F
-
recombinant overexpression in Escherichia coli
-
sequence comparisons