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4.1.99.3: deoxyribodipyrimidine photo-lyase

This is an abbreviated version!
For detailed information about deoxyribodipyrimidine photo-lyase, go to the full flat file.

Word Map on EC 4.1.99.3

Reaction

cyclobutadipyrimidine (in DNA)
= 2 pyrimidine residues (in DNA)

Synonyms

AMV025, AnPL, AtCry1, AtCry3, AtPL, BcCRY1, BcCRY2, BCIN_05g08060, BCIN_09g01620, BsUvrC, Cc-phr2, CcPL, CDP photolyase, class I CPD photolyase, class I cyclobutane pyrimidine dimer photolyase, class I photolyase, class I PL, class II AtPL, class II CPD photolyase, class II CPD-DNA photolyase, class II CPD-photolyase, class II cyclobutane pyrimidine dimer photolyase, class II DmPL, class II DNA photolyase, class II photolyase, class II PL, class III PL, cold-adapted DNA photolyase, CPD class I photolyase, CPD photolyase, CPD photolyase photolyase, CPD specific photolyase, CPD-class I photolyase, CPD-photolyase, CPD-Phr, CPD-specific DNA photolyase, CPD1, CPD2PHR, CPDI, CPDII, CPDphr, CPH1, CPH1-PHR, CpPL, Cry-DASH, Cry1, Cry2, cry3, CryA, CRYD, cryptochrome 1, cryptochrome 3, CRYPTOCHROME DASH, cryptochrome-3, cryptochrome-DASH, CsPHR, cyclobutane pyrimidine dimer photolyase, cyclobutane pyrimidine dimer PHR, cyclobutane pyrimidine dimer specific photolyase, cyclobutane pyrimidine dimer-class I photolyase, cyclobutane pyrimidine dimer-specific DNA photolyase, cyclobutane pyrimidine dimers photolyase, CYME_CMA044C, cytochrome DASH, DASH cryptochrome, deoxyribodipyrimidine photolyase, deoxyribodipyrimidine photolyase type I enzyme, deoxyribonucleate pyrimidine dimer lyase (photosensitive), deoxyribonucleic cyclobutane dipyrimidine photolyase, deoxyribonucleic photolyase, dipyrimidine photolyase (photosensitive), DmPL, DNA cyclobutane dipyrimidine photolyase, DNA photolyase, DNA photolyase type I enzyme, DNA repair protein photolyase, DNA-photoreactivating enzyme, EcPL, eukaryotic Class II CPD PHR, eukaryotic class II cyclobutane pyrimidine dimer photolyase, lyase, deoxyribonucleate pyrimidine dimer, MmCPDII, MmPL, MM_0852, photolyase, photolyase I, photolyase orthologue, photoreactivating enzyme, PHR, phr A photolyase, PHR1, PHR2, phrA, PhrB, PhrB orthologue, PhrB photolyase, PL, PRE, primase, Saci_1227, ssDNA, ssDNA AtCRY3, ssDNA photolyase, ssDNA PL, SsPL, St-photolyase, tetur12g04440, tetur12g04460, tetur35g00010, tetur35g00030, thymine dimer by photolyase, VcCry1, VcPHR, Ver3Phr, VPA1471, XlCry-DASH

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.3 deoxyribodipyrimidine photo-lyase

Cloned

Cloned on EC 4.1.99.3 - deoxyribodipyrimidine photo-lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
6xHis-tagged protein expressed in Escherichia coli
apophotolyase (devoid of the antenna cofactor 5,10-methenyltetrahydrofolate) overproduced in Escherichia coli
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AtCry1 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to possess no photorepair activity under any condition
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AtCry3 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA
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BsUvrC belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to possess no photorepair activity under any condition
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cloned and overexpressed in Escherichia coli by using plasmid pMS969 (TetR AmpR Phr+), a derivative of ptac12 and propagation in any RecA- strain carrying Flac iQ. Optimal levels of expression are obtained using freshly transformed cells. Single colony is inoculated into 5 ml of LB and incubated at 37°C with shaking for 8 h. 1 ml of preculture is used to prepare an overnight culture consisting of 100 ml of LB. 10 ml of the overnight culture are added to 1 liter of LB and incubated with vigorous shaking at 37°C until the A600: 0.6-0.8 (3.5-4 h), at which time 2.0 ml of 0.5 M IPTG is added. Incubation is continued for another 4 h, at which point the photolyase typically constitutes 10-15% of total cellular protein
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cloned and overexpressed in Escherichia coli CSR603 (recA1 uvrA6 phr1) containing F’laciQ using plasmid pCB1241 carrying the DNA photolyase gene under the control of the tac promoter on a TetR derivative of pUNC09. For overexpression, 40 ml LB containing 20 g/ml of tetracycline with 0.4 ml of the stock culture is inoculated and incubate at 37°C for 12–16 h with shaking. Four 1 liter cultures with 10 ml of the overnight culture are inoculated and incubated until the culture reaches A600: 0.8–1.0. IPTG is added to a final concentration of 1 mM, incubation for further 12–16 h.
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cloned in Escherichia coli
cloned in Escherichia coli as a C-terminal His-tagged fusion protein
coding region of the CPD photolyase gene under the control of the CaMV 35S tandem promoter placed together with a hygromycin resistance gene on the T-DNA of a binary vector and overexpressed in transgenic Arabidopsis lines
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis
DNA and amino acid sequence determination and analysis, seven genes displaying homology to the CPF in Cyanidioschyzon merolae, an extremophilic microalga. Characterization of these seven putative CPF members identifies three genes encoding CRY-DASHs, accordingly named as CmPHR2, CmPHR5 and CmPHR6
expressed from Escherichia coli strain UNC523F transfected with the pUNC2002 plasmid
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expressed in a photolyase-deficient Escherichia coli strains NKJ3002 and KY20
expressed in Arabidopsis thaliana
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expressed in DANN-repair deficient Escherichia coli strains DH5alpha and MACH-1
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expressed in Escherichia coli
expressed in Escherichia coli Codon Plus BL21[pREP4] cells
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expressed in Escherichia coli strain BL21(DE3)
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expressed in Escherichia coli strain KY29
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expressed in Escherichia coli strain UNC523
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expressed in Escherichia coli, the purified native rice CPD photolyase has significantly higher CPD photorepair activity than the Escherichia coli-expressed CPD photolyase, the structure of the native rice CPD photolyase differs significantly from that of the Escherichia coli-expressed rice CPD photolyase, and the structural modification of the native CPD photolyase leads to higher activity
expressed in MS09 cells
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expressed in SV-40 transformed human fibroblasts
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expression in Escherichia coli
expression in transgenic mice
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fusion protein with maltose-binding protein
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fusion protein with maltose-binding protein, expressed in Escherichia coli
gene bccry1, light-induced gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression of GFP-tagged enzyme BcCRY1-GFP in Botrytis cinerea, it restores the wild-type phenotype in the BcCRY1 deletion mutant
gene bccry2, light-induced gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression of GFP-tagged enzyme GFP-BcCRY2 in Botrytis cinerea, it restores the wild-type phenotype in the BcCRY2 deletion mutant
gene CL42_09140, DNA and amino acid equence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), expression of the enzyme Ver3Phr in and functional complementation of an Escherichia coli strain KY1225 deficient in photolyase
gene phr, expression of wild-type and mutant enzymes from vector pTD1 vector by in vitro translation using a cell-free insect system
gene phr2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), DNA photolyase increases photo-repair more than 116fold in Escherichia coli repair-deficient strain SY2 under 0.1 mw/cm2UVB radiation
gene phrB, overexpression in MS09 cells
gene phrB, recombinant expression in Escherichia coli enzyme-deficient mutant strain CSR603. The Neisseria gonorrhoeae phrB cannot complement an Escherichia coli phrB mutant strain CSR603
gene phrB, recombinant expression in Escherichia coli strain Rosetta 2 (DE3)
gene VPA1471 or phr, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, functional recombinant expression of His6-tagged enzyme in Escherichia coli from pET-30a vector
genes phr1 and phr2, transient expression of C-terminally EGFP-tagged PHR1 and 2 in HEK 293T cells using vector pcDNA3.1, cotransfection with empty vector, Flag-Clock, or Flag-Bmal1
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genome of the facultatively photosynthetic bacterium Rhodobacter sphaeroides encodes three proteins of the photolyase/cryptochrome family. It is shown that phrA (RSP2143) encodes a functional photolyase, which is an enzyme that repairs UV radiation-induced DNA damage in a blue light dependent manner
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GST-tagged class II CPD photolyase overexpressed in Escherichia coli
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holophotolyase (both flavin and antenna cofactor 5,10-methenyltetrahydrofolate present) overexpressed
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N-terminally His6-tagged fusion protein in Escherichia coli
overexpression in Escherichia coli
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overexpression of the enzyme from VcPhr genes in pmal-c2x bacterial expression vector in Escherichia coli strain UNC 523
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phr gene is expressed under the control of a tac promoter on plasmid pUNC1993 in Escherichia coli strain CSR603 F’laciQ. Conditions are similar as expression for the enzyme derived from Escherichia coli. With the exception that induction is with 1 mM IPTG and postinduction incubation at 37°C continues for 10 h prior to harvesting the cells.
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phr1 fragment subcloned between the HindIII and BamHI sites located downstream of the Orgyia pseudotsugata multiple NPV immediate early 2 promoter and upstream of the EGFP reporter gene in the vector PIZ-EGFP-N3 to generate PIZ-phr1-EGFP. Coding region of the phr2 gene flanked by EcoRI and BamHI restriction sites amplified and cloned into PIZ-EGFP-N3 between the corresponding restriction sites to give PIZ-phr2-EGFP. Complete ORF of phr2 with stop codon and flanked by EcoRI sites recloned from a phr2-containing pGEM-T Easy vector into the EcoRI site of PIZ/HIS-V5. Expression of phr2-egfp and non-fused phr2 in transfected Trichoplusia ni High Five cells
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phylogenetic analysis
PriL-CTD construct where the 381-RNG-383 sequence is excised, expressed in Escherichia coli strain BL21(DE3) Rosetta2. Full-length and C-terminally truncated primase
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recombinant expression of CpPL in Escherichia coli BL21(DE3) in inclusion bodies, as His6-tagged protein in Escherichia coli Arctic Express (DE3) cells, and as MBP-fusion protein in Escherichia coli strain BL21. Recombinant CpPL (rCpPL) binds two different second cofactor molecules, flavin mononucleotide (FMN) when overexpressed and purified from Escherichia coli BL21 (DE3) inclusion bodies, and a folate (possibly MTHF) when overexpressed and purified from Escherichia coli Arctic Express (DE3) cells as a His6-tagged protein or in strain BL21(DE3) cells as a MBP-fusion protein
recombinant expression of GST-tagged enzyme
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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recombinant overexpression in Escherichia coli
Tetranychus urticae has four copies of the CPD photolyase gene (tetur12g04440, tetur12g04460, tetur35g00010 and tetur35g00030), all of which are highly homologous to each other, cloning and quantitative real-time RT-PCR expression analysis
transgenic rice plants bearing the CPD photolyase gene of the UV-resistant rice cultivar Sasanishiki in the sense and antisense orientation are generated
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unrooted phylogenetic tree
untagged photolyase overexpressed from pET3a vector in Escherichia coli BL21
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VcCry1 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA
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when a photolyase-deficient Escherichia coli strain is transformed with the cDNA, photoactivation activity is partially restored
wild-type and mutant
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wild-type and mutants cloned into pET3a vectors and overexpressed in Escherichia coli BL21(DE3)pLysS cells
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XlCry-DASH belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA
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