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4.1.99.22: GTP 3',8-cyclase

This is an abbreviated version!
For detailed information about GTP 3',8-cyclase, go to the full flat file.

Word Map on EC 4.1.99.22

Reaction

GTP
+
S-adenosyl-L-methionine
+
reduced electron acceptor
=
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate
+
5'-deoxyadenosine
+
L-methionine
+
oxidized electron acceptor

Synonyms

AT2G31955, cnx2, CNX2/MOCS1A, EC 4.1.99.18, MoaA, MoaC, Moco-biosynthesis protein, MOCS1A, MOCS1B, MogA, molybdenum cofactor biosynthetic enzyme, molybdenum-cofactor biosynthesis protein

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.22 GTP 3',8-cyclase

Purification

Purification on EC 4.1.99.22 - GTP 3',8-cyclase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified under anaerobic conditions
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by two different times of gel filtration and anion exchange chromatography, followed by gel filtration and hydroxyapatite chromatography, and a last step of gel filtration
recombinant MOCS1B from Escherichia coli strain BL21(DE3) by streptomycin sulfate and ammonium sulfate precipitation steps, nickel affinity chromatography, gell filtration, and ultrafiltration
-