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4.1.99.22: GTP 3',8-cyclase

This is an abbreviated version!
For detailed information about GTP 3',8-cyclase, go to the full flat file.

Word Map on EC 4.1.99.22

Reaction

GTP
+
S-adenosyl-L-methionine
 +
reduced electron acceptor
=
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate
 +
5'-deoxyadenosine
 +
L-methionine
 +
oxidized electron acceptor

Synonyms

AT2G31955, cnx2, CNX2/MOCS1A, EC 4.1.99.18, MoaA, MoaC, Moco-biosynthesis protein, MOCS1A, MOCS1B, MogA, molybdenum cofactor biosynthetic enzyme, molybdenum-cofactor biosynthesis protein

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.22 GTP 3',8-cyclase

Crystallization

Crystallization on EC 4.1.99.22 - GTP 3',8-cyclase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme, mixing of 0.001 ml of 24 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 0.2 M NaCl, with 0.001 ml of reservoir solution containing 40% v/v PEG 600, 100 mM CHES buffer, pH 9.5, or 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG 3350, 1 week, two different crystal forms, X-ray diffraction structure determination and analysis at 1.7-1.9 A resolution, molecular replacement method
crystal structure of wild-type MoaA, MoaA-R17A/R266A/R268A and MoaA in complex with 5'-GTP2.35 A resolution
crystals are grown under anaerobic conditions, hanging drop vapor diffusion technique, crystals belong to space group P2(1)2(1)2(1) with cell dimensions of a = 48.1, b = 102.4, and c = 191.2 A and contain two molecules per asymmetric unit, structures of MoaA in the apo-state (2.8 A) and in complex with S-adenosyl-L-methionine (2.2 A)
purified recombinant enzyme, X-ray diffraction structure determination and analysis at 1.64 A resolution, molecular replacement method