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4.1.99.13: (6-4)DNA photolyase

This is an abbreviated version!
For detailed information about (6-4)DNA photolyase, go to the full flat file.

Word Map on EC 4.1.99.13

Reaction

(6-4) photoproduct (in DNA)
= 2 pyrimidine residues (in DNA)

Synonyms

(6-4) DNA photolyase, (6-4) photolyase, (6-4) PHR, (6-4) PL, (6-4) PP-specific PL, (6-4)-Phr, (6-4)photolyase, 6-4 DNA photolyase, 6-4 photolyase, 6-4CiPhr, 6-4PP-photolyase, animal (6-4) photolyase, At64, At64PHR, bacterial (6-4) photolyase, CmPHR1, Cry1, CryB, deoxyribodipyrimidine photolyase-related protein, Dm64, DNA photolyase, Ds64PHR, H64PRH, human (6-4) photolyase homologous protein, NF-10, OtCPF1, phr (6-4), PhrB, PL-(6-4), prokaryotic (6-4) photolyase, RSP_3077, TRIREDRAFT_77473, XELAEV_18035355mg, Xl64phr

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.13 (6-4)DNA photolyase

Cloned

Cloned on EC 4.1.99.13 - (6-4)DNA photolyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a cDNA of (6-4)photolyase from Dunaliella salina is cloned, sequenced and its amino acid sequence is deduced
cloned and overexpressed in Escherichia coli
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DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis
due to insolubility problems (6-4) photolyases is overexpressed as a fusion protein in Escherichia coli. Plasmid pXZ1997, a derivative of pMal-c2 containing the Drosophila melanogaster phr(6-4) cDNA fused in frame to the malE gene encoding maltose-binding protein (MBP), is propagated in Escherichia coli strain UNC523 (phr::kan uvrA::Tn10) selecting for ampicillin resistance. Cells are cultured in 2 liter of LB to A600: 0.6–0.8. IPTG is added to 0.3 mM, and incubation continued for 6 h prior to harvesting the cells by centrifugation.
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expressed as a GST-fusion protein in Escherichia coli
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expressed as a GST-tagged fusion protein in Escherichia coli
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expressed as a GSTtagged fusion protein in Escherichia coli
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expressed as a His-tagged fusion protein
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expressed in Escherichia coli
expressed in Escherichia coli as a GST-fusion protein
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expressed in Escherichia coli as a MBP-PL-(6-4) fusion protein
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expressed in Escherichia coli, fusion protein with glutathione S-transferase
expression in Escherichia coli
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene 6-4CiPhr, transcriptome analysis and cloning, DNA and amino acid sequence determination and analysis, phylogenetic tree, real-time PCR enzyme expression analysis, functional recombinant expression of C-terminally His-tagged enzyme in a photolyase-deficient Escherichia coli strain
gene cry1, sequence comparisons and phylogenetic analysis
gene phrB, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain ER2566
gene RSP_3077 or cryB, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains BL21(DE3)pLysS or Rosetta(DE3)pRARE
overexpressed in Escherichia coli
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phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression of isozymes in Escherichia coli strain UNC 523 from pMal-c2x vector
recombinant expression of GST-tagged enzyme in Escherichia coli, and recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain C41(DE3). Photorepair difference FTIR spectra of Xenopus laevis (6-4) PHR using His-tagged sample and GST-tagged sample, overview
recombinant expression of His-tagged enzyme in Escherichia coli
recombinant expression of wild-type and mutant enzymes in Escherichia coli
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recombinant protein expressed in Escherichia coli
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