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4.1.99.11: benzylsuccinate synthase

This is an abbreviated version!
For detailed information about benzylsuccinate synthase, go to the full flat file.

Word Map on EC 4.1.99.11

Reaction

benzylsuccinate
=
Toluene
+
fumarate

Synonyms

(R)-benzylsuccinate synthase, benzylsuccinate synthase, benzylsuccinate synthase A, BSS, BSSA, toluene-activating enzyme

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.11 benzylsuccinate synthase

Cloned

Cloned on EC 4.1.99.11 - benzylsuccinate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli as a Strep-tagged fusion protein
-
expression in Escherichia coli BL21DE3 cells, the vectors pET-28b, pACYC-Duet, pET-Duet and pRSF-Duet are used
-
gene bssA, phylogenetic analysis
gene order of all known bss operons is bssDCAB, starting with the gene for the activating enzyme and followed by those for the gamma-, alpha- and beta-subunits, phylogenetic analysis
recombinant enzyme expression in Escherichia coli strain BL21(DE3)