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2-oxindolyl-L-alanine + H2O
?
-
-
-
?
4-methyl-Trp + H2O
?
-
52% of the activity with L-Trp
-
-
?
5-hydroxyindole + L-Cys
5-hydroxy-L-Trp + ?
-
-
-
-
?
5-hydroxyindole + S-methyl-L-Cys
5-hydroxy-L-Trp + ?
-
-
-
-
?
5-methylindole + L-Cys
5-methyl-L-Trp + ?
-
-
-
-
?
5-methylindole + S-methyl-L-Cys
5-methyl-L-Trp + ?
-
-
-
-
?
6-methyl-Trp + H2O
?
-
44% of the activity with L-Trp
-
-
?
alpha,beta-diaminopropionic acid
?
-
-
-
-
?
beta-(benzimidazol-1-yl)-L-alanine + H2O
?
beta-chloro-L-Ala + H2O
?
beta-chloroalanine + H2O
?
beta-chloroalanine + H2O
pyruvate + NH4+ + Cl-
-
-
-
-
?
cysteine sulfinic acid + H2O
?
-
-
-
-
?
D-serine
pyruvate + NH3
-
with high diammonium hydrogen phosphate concentration, e.g. 1.2 M
-
-
?
D-serine + indole
L-tryptophan + H2O
D-Trp + H2O
indole + pyruvate + NH4+
indole + L-Ser
L-Trp + ?
-
-
-
?
indole + pyruvate + NH4+
L-Trp + H2O
indole + S-methyl-L-Cys
L-Trp + ?
L-Ser + H2O
pyruvate + H2O
-
-
-
-
?
L-serine + indole
L-tryptophan + H2O
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH3
-
effects of temperature and hydrostatic pressure on the equilibria and rate constants for quinoid intermediate formation from L-Trp and L-Met with H463 mutant enzyme
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
L-Trp + H2O
pyruvate + NH3 + indole
L-tryptophan + H2O
indole + pyruvate + NH3
O-benzylserine + H2O
?
-
-
-
-
?
O-methylserine + H2O
?
-
-
-
-
?
oxindolyl-L-alanine + H2O
?
OIA, transition state analogue, the reaction of enzyme TIL mutant Y72F with OIA is accompanied by the appearance of intense absorption at 509 nm which may be explained by the formation of a quinonoid intermediate, transformation of the external aldimine into the quinonoid intermediate implies the transfer of alpha-proton to the base-acceptor in the active site. Oxindolyl-L-alanine also inhibits the enzyme
-
-
?
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
S-(2-nitrophenyl)-L-cysteine + H2O
?
-
-
-
-
r
S-(o-nitrophenyl)-L-cysteine
?
-
-
-
?
S-benzyl-L-Cys + H2O
phenylmethanethiol + pyruvate + NH4+
S-benzyl-L-cysteine + H2O
phenylmethanethiol + pyruvate + NH3
S-benzylcysteine + H2O
pyruvate + NH4+ + phenylmethanethiol
-
-
-
-
?
S-ethyl-L-Cys + H2O
ethanethiol + pyruvate + NH4+
S-ethyl-L-cysteine + H2O
?
S-ethyl-L-cysteine + H2O
ethanethiol + pyruvate + NH3
S-ethyl-L-cysteine + H2O
ethanethiol + pyruvate + NH4+
S-ethyl-L-cysteine + H2O
pyruvate + NH4+ + ethanethiol
-
-
-
-
?
S-ethylcysteine + H2O
?
-
-
-
-
?
S-methyl-L-Cys + H2O
methanethiol + pyruvate + NH4+
S-methyl-L-Cys + H2O
pyruvate + NH4+ + methanethiol
-
-
-
-
?
S-methyl-L-Cys + indole
Trp + ?
S-methyl-L-cysteine
?
-
-
-
?
S-methyl-L-cysteine + H2O
?
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH3
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH4+
S-o-nitrophenyl-L-Cys + H2O
o-nitrothiophenol + pyruvate + NH3
S-o-nitrophenyl-L-Cys + indole
Trp + ?
-
reaction readily proceeds in water, it becomes impossible in water-organic solvents
-
?
S-o-nitrophenyl-L-cysteine + H2O
o-nitrothiophenol + pyruvate + NH4+
-
-
-
-
?
additional information
?
-
5-hydroxy-L-Trp + H2O
?
-
6% of the activity with L-Trp
-
-
?
5-hydroxy-L-Trp + H2O
?
-
14.3% of the activity with L-Trp
-
-
?
5-hydroxy-L-Trp + H2O
?
-
6.7% of the activity with L-Trp
-
-
?
5-hydroxy-L-Trp + H2O
?
-
6.7% of the activity with L-Trp
-
-
?
5-methyl-L-Trp + H2O
?
-
22% of the activity with L-Trp
-
-
?
5-methyl-L-Trp + H2O
?
-
21.1% of the activity with L-Trp
-
-
?
5-methyl-L-Trp + H2O
?
-
15.2% of the activity with L-Trp
-
-
?
5-methyl-L-Trp + H2O
?
-
15.2% of the activity with L-Trp
-
-
?
beta-(benzimidazol-1-yl)-L-alanine + H2O
?
-
reaction via aldimine intermediate, high activity with wild-type enzyme and mutant H463F
-
-
?
beta-(benzimidazol-1-yl)-L-alanine + H2O
?
-
reaction via aldimine intermediate, high activity with wild-type enzyme and mutant H463F
-
-
?
beta-chloro-L-Ala + H2O
?
-
-
-
-
?
beta-chloro-L-Ala + H2O
?
-
-
-
?
beta-chloro-L-Ala + H2O
?
-
-
-
?
beta-chloroalanine + H2O
?
-
-
-
-
?
beta-chloroalanine + H2O
?
-
-
-
-
?
Cys + H2O
?
-
-
-
-
?
Cys + H2O
?
-
36.4% of the activity with L-Trp
-
-
?
Cys + H2O
?
-
L-Cys
-
-
?
Cys + H2O
?
-
L-Cys
-
-
?
Cys + H2O
?
-
18.9% of the activity with L-Trp
-
-
?
Cys + H2O
?
-
L-Cys
-
-
?
Cys + H2O
?
-
18.9% of the activity with L-Trp
-
-
?
Cys + H2O
?
-
L-Cys
-
-
?
Cys + indole
Trp + ?
-
-
-
?
Cys + indole
Trp + ?
-
-
-
?
Cys + indole
Trp + ?
-
-
-
?
D-serine + indole
L-tryptophan + H2O
-
the presence of 20% saturation concentration of diammonium hydrogen phosphate is required for this reaction
-
-
?
D-serine + indole
L-tryptophan + H2O
-
with high diammonium hydrogen phosphate concentration, e.g. 1.2 M
-
-
?
D-Trp + H2O
indole + pyruvate + NH4+
in the presence of high concentrations of ammonium phosphate
-
-
?
D-Trp + H2O
indole + pyruvate + NH4+
in the presence of high concentrations of ammonium phosphate
-
-
?
indole + L-Cys
L-Trp + ?
-
-
-
?
indole + L-Cys
L-Trp + ?
-
beta-replacement reaction
-
?
indole + L-Cys
L-Trp + ?
-
beta-replacement reaction
-
?
indole + pyruvate + NH4+
L-Trp + H2O
-
-
-
?
indole + pyruvate + NH4+
L-Trp + H2O
-
-
-
?
indole + pyruvate + NH4+
L-Trp + H2O
-
-
-
?
indole + pyruvate + NH4+
L-Trp + H2O
-
-
-
?
indole + S-methyl-L-Cys
L-Trp + ?
-
-
-
?
indole + S-methyl-L-Cys
L-Trp + ?
-
beta-replacement reaction
-
?
indole + S-methyl-L-Cys
L-Trp + ?
-
beta-replacement reaction
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
Escherichia aurescens
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
Escherichia aurescens
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
Escherichia aurescens
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
r
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
r
L-Trp + H2O
indole + pyruvate + NH4+
-
the product indole is involved in cell signaling, involved in cell cycle regulation
-
-
r
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
r
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
Paracolobactrum coliforme
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
Paracolobactrum sp.
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
Shigella alkalescens
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
Shigella alkalescens
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
Shigella alkalescens
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
indole + pyruvate + NH4+
-
-
-
?
L-Trp + H2O
pyruvate + NH3 + indole
-
-
-
-
?
L-Trp + H2O
pyruvate + NH3 + indole
-
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
-
active to D-tryptophan in highly concentrated diammonium hydrogen phosphate solution
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
reversible hydrolytic beta-elimination
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
Tnase can act in reverse to form L-tryptophan at high concentrations of pyruvate and ammonia
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
very high substrate specificity of TIL
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
r
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
very high substrate specificity of TIL
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
?
L-tryptophan + H2O
indole + pyruvate + NH3
-
-
-
?
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
-
-
-
-
?
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
-
-
-
-
ir
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
-
-
-
?
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
-
-
-
?
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
-
-
-
?
S-(2-nitrophenyl)-L-cysteine + H2O
2-nitrobenzenethiolate + pyruvate + NH3
-
-
-
?
S-benzyl-L-Cys + H2O
phenylmethanethiol + pyruvate + NH4+
-
-
-
-
?
S-benzyl-L-Cys + H2O
phenylmethanethiol + pyruvate + NH4+
-
-
-
?
S-benzyl-L-Cys + H2O
phenylmethanethiol + pyruvate + NH4+
-
-
-
?
S-benzyl-L-cysteine + H2O
phenylmethanethiol + pyruvate + NH3
-
-
-
?
S-benzyl-L-cysteine + H2O
phenylmethanethiol + pyruvate + NH3
-
-
-
?
S-benzylcysteine + H2O
?
-
-
-
-
?
S-benzylcysteine + H2O
?
-
-
-
-
?
S-ethyl-L-Cys + H2O
ethanethiol + pyruvate + NH4+
-
-
-
-
?
S-ethyl-L-Cys + H2O
ethanethiol + pyruvate + NH4+
-
-
-
?
S-ethyl-L-Cys + H2O
ethanethiol + pyruvate + NH4+
-
-
-
?
S-ethyl-L-cysteine + H2O
?
-
-
-
-
?
S-ethyl-L-cysteine + H2O
?
-
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + pyruvate + NH3
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + pyruvate + NH3
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + pyruvate + NH3
S-ethyl-L-cysteine represents a substrate with a better leaving group as compared to L-tryptophan
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + pyruvate + NH4+
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + pyruvate + NH4+
-
-
-
?
S-methyl-L-Cys + H2O
?
-
-
-
-
?
S-methyl-L-Cys + H2O
?
-
-
-
-
?
S-methyl-L-Cys + H2O
?
-
-
-
-
?
S-methyl-L-Cys + H2O
?
-
31.2% of the activity with L-Trp
-
-
?
S-methyl-L-Cys + H2O
?
-
-
-
-
?
S-methyl-L-Cys + H2O
?
-
63.8% of the activity with L-Trp
-
-
?
S-methyl-L-Cys + H2O
?
-
63.8% of the activity with L-Trp
-
-
?
S-methyl-L-Cys + H2O
methanethiol + pyruvate + NH4+
-
-
-
-
?
S-methyl-L-Cys + H2O
methanethiol + pyruvate + NH4+
-
-
-
?
S-methyl-L-Cys + indole
Trp + ?
-
-
-
-
?
S-methyl-L-Cys + indole
Trp + ?
-
-
-
?
S-methyl-L-Cys + indole
Trp + ?
-
-
-
?
S-methyl-L-Cys + indole
Trp + ?
-
-
-
-
?
S-methyl-L-Cys + indole
Trp + ?
-
-
-
?
S-methyl-L-cysteine + H2O
?
-
-
-
-
?
S-methyl-L-cysteine + H2O
?
-
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH3
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH3
-
-
-
r
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH3
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH3
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH4+
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + pyruvate + NH4+
-
-
-
?
S-o-nitrophenyl-L-Cys + H2O
o-nitrothiophenol + pyruvate + NH3
-
-
-
?
S-o-nitrophenyl-L-Cys + H2O
o-nitrothiophenol + pyruvate + NH3
-
-
-
-
?
S-o-nitrophenyl-L-Cys + H2O
o-nitrothiophenol + pyruvate + NH3
-
-
-
?
S-o-nitrophenyl-L-Cys + H2O
o-nitrothiophenol + pyruvate + NH3
-
-
-
-
?
S-o-nitrophenyl-L-Cys + H2O
o-nitrothiophenol + pyruvate + NH3
-
-
-
-
?
Ser + H2O
?
-
-
-
-
?
Ser + H2O
?
-
4.6% of the activity with L-Trp
-
-
?
Ser + H2O
?
-
L-Ser
-
-
?
Ser + H2O
?
-
5.3% of the activity with L-Trp
-
-
?
Ser + indole
Trp + ?
-
-
-
-
?
Ser + indole
Trp + ?
-
-
-
?
Ser + indole
Trp + ?
-
-
-
?
Ser + indole
Trp + ?
-
-
-
-
?
Ser + indole
Trp + ?
-
-
-
?
Ser + indole
Trp + H2O
-
-
-
-
?
Ser + indole
Trp + H2O
-
-
-
-
?
additional information
?
-
-
induced by Trp
-
-
?
additional information
?
-
-
tryptophanase displays no activity on D-tryptophan under usual conditions. However, it becomes active toward D-tryptophan degradation in highly concentrated diammonium hydrogen phosphate solutions
-
-
?
additional information
?
-
-
synthesis of L-tryptophan from L-cysteine, pyridoxal 5'-phosphate, and indole by the recombinant enzyme expressed in Escherichia coli strain BL21(DE3)
-
-
?
additional information
?
-
only the correctly protonated form of the enzyme binds the substrate, and the enzyme-substrate complex does not undergo protonation
-
-
?
additional information
?
-
-
tryptophanase does not degrade L-alanine, L-serine or L-cysteine
-
-
?
additional information
?
-
-
tryptophanase does not degrade L-alanine, L-serine or L-cysteine
-
-
?
additional information
?
-
the enzyme is capable to catalyze alpha,beta-elimination reactions with a number of other amino acids, containing better leaving groups compared to L-tryptophan, e.g. S-ethyl-L-cysteine. For the wild-type enzyme the reactions with substrates are described by the same kinetic scheme where binding of holoenzyme with an amino acid, leading to reversible formation of an external aldimine, proceeds very fast, while following transformations, leading finally to reversible formation of a quinonoid intermediate proceed with measureable rates
-
-
?
additional information
?
-
-
the enzyme is capable to catalyze alpha,beta-elimination reactions with a number of other amino acids, containing better leaving groups compared to L-tryptophan, e.g. S-ethyl-L-cysteine. For the wild-type enzyme the reactions with substrates are described by the same kinetic scheme where binding of holoenzyme with an amino acid, leading to reversible formation of an external aldimine, proceeds very fast, while following transformations, leading finally to reversible formation of a quinonoid intermediate proceed with measureable rates
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
inducible enzyme
-
-
?
additional information
?
-
coupled assay: the decrease in NADH absorbance at 340 nm in the presence of LDH is used to determine Trpase activity after adding the substrate. L-Phenylalanine and L-serine are no substrates
-
-
?
additional information
?
-
coupled assay: the decrease in NADH absorbance at 340 nm in the presence of LDH is used to determine Trpase activity after adding the substrate. L-Phenylalanine and L-serine are no substrates
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
the enzyme is induced by Trp and its analogs, and partially repressed by 0.5% glucose or glycerol
-
-
?
additional information
?
-
-
the enzyme is induced by Trp and its analogs, and partially repressed by 0.5% glucose or glycerol
-
-
?