4.1.3.6: citrate (pro-3S)-lyase

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For detailed information about citrate (pro-3S)-lyase, go to the full flat file.

Reaction

Synonyms

citrase, citratase, citrate (pro-3S)-lyase, citrate aldolase, citrate lyase, citrate oxaloacetate-lyase(pro-3S-COO- --> acetate), citric aldolase, citridesmolase, citritase, lyase, citrate, More

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.3 Oxo-acid-lyases

                4.1.3.6 citrate (pro-3S)-lyase

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Results	in table
6	Activating Compound
8202	AA Sequence
4	Application
1	CAS Registry Number
5	Cloned(Commentary)
3	Cofactor
1	Crystallization (Commentary)
2	Disease/ Diagnostics
8	Expression
2	General Information
4	General Stability
15	Inhibitors
6	KM Value [mM]
53	Metals/Ions
60	Molecular Weight [Da]
62	Natural Substrates/ Products (Substrates)
78	Organism
11	PDB ID
10	pH Optimum
1	pH Range
7	Posttranslational Modification
11	Purification (Commentary)
1	Reaction
2	Reaction Type
78	Reference
9	Source Tissue
24	Specific Activity [micromol/min/mg]
15	Storage Stability
128	Substrates and Products (Substrate)
22	Subunits
31	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]

Storage Stability

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Storage Stability on EC 4.1.3.6 - citrate (pro-3S)-lyase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, stable for 2 months

Lacrimispora sphenoides

-

33362, 33363

-20°C, stable for at least 6 months

Klebsiella pneumoniae

-

33372

-90°C, stable

Lactococcus lactis subsp. lactis

-

33363

0°C or -20°C, stable for at least 2-3 days

Escherichia coli

-

33364

0°C or -20°C, stable in presence of ammonium sulfate

Enterococcus faecalis

-

33357, 33363

0°C, 30% glycerol, 40% sucrose or 10 mg per ml bovine serum albumin, 7 mM L-Glu, only some loss of activity after 5 min

Lacrimispora sphenoides

-

33362

0°C, in presence of saturated ammium sulfate and MgSO4, stable

Escherichia coli

-

33363

4°C, crystals suspended in 3 M ammonium sulfate, stable for 8 months

Rubrivivax gelatinosus

-

33360, 33363

4°C, pH 7.2, 100 mM potassium phosphate buffer, 3 mM MgCl2, 1 mM dithiothreitol, stable for 3 days

[Clostridium] sporosphaeroides

-

698

4°C, stable for 4 days

Lacrimispora sphenoides

-

33362, 33363

4°C, stable for at least 1 month

Klebsiella pneumoniae

-

33372