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4.1.3.44: tRNA 4-demethylwyosine synthase (AdoMet-dependent)

This is an abbreviated version!
For detailed information about tRNA 4-demethylwyosine synthase (AdoMet-dependent), go to the full flat file.

Word Map on EC 4.1.3.44

Reaction

N1-methylguanine37 in tRNAPhe
+
pyruvate
 +
S-adenosyl-L-methionine
=
4-demethylwyosine37 in tRNAPhe
 +
L-methionine
 +
5'-deoxyadenosine
 +
CO2
 +
H2O

Synonyms

4-demethylwyosine synthase, Taw1, tRNA wyosine derivatives biosynthesis protein Taw1, TYW1, YPL207W

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.44 tRNA 4-demethylwyosine synthase (AdoMet-dependent)

Purification

Purification on EC 4.1.3.44 - tRNA 4-demethylwyosine synthase (AdoMet-dependent)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, butyl Sepharose column chromatography, and HiTrap chelating column chromatography
ammonium sulfate precipitation, HiTrap chealting column chromatography, and butyl Sepharose column chromatography
-
purification and reconstitution of recombinant protein
purified anaerobically and reconstituted with Fe and S. The reconstituted protein is brown and has an absorbance spectrum that displays a characteristic shoulder at 400 nm