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4.1.3.44: tRNA 4-demethylwyosine synthase (AdoMet-dependent)

This is an abbreviated version!
For detailed information about tRNA 4-demethylwyosine synthase (AdoMet-dependent), go to the full flat file.

Word Map on EC 4.1.3.44

Reaction

N1-methylguanine37 in tRNAPhe
+
pyruvate
 +
S-adenosyl-L-methionine
=
4-demethylwyosine37 in tRNAPhe
 +
L-methionine
 +
5'-deoxyadenosine
 +
CO2
 +
H2O

Synonyms

4-demethylwyosine synthase, Taw1, tRNA wyosine derivatives biosynthesis protein Taw1, TYW1, YPL207W

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.44 tRNA 4-demethylwyosine synthase (AdoMet-dependent)

Engineering

Engineering on EC 4.1.3.44 - tRNA 4-demethylwyosine synthase (AdoMet-dependent)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C195S
the mutant shows reduced activity compared tot he wild type enzyme
C195S
-
the mutant shows reduced activity compared tot he wild type enzyme
-
C444S
loss of activity
C457S
loss of activity
C470S
loss of activity
C479A
mutation in the conserved C479xxxC483xxC486 motif of the radical-Ado-Met domain, complete loss of activity
C479S
loss of activity
C483A
mutation in the conserved C479xxxC483xxC486 motif of the radical-Ado-Met domain, complete loss of activity
C483S
loss of activity
C486A
mutation in the conserved C479xxxC483xxC486 motif of the radical-Ado-Met domain, complete loss of activity
C486S
loss of activity
D597A
loss of activity
D597N
loss of activity
E532A
mutation in a candidate GGE motif in the radical Ado-Met domain, no loss of activity
E550A
K459A
loss of activity
R638A
loss of activity
S595A
loss of activity
C457S
-
loss of activity
-
C470S
-
loss of activity
-
C479A
-
mutation in the conserved C479xxxC483xxC486 motif of the radical-Ado-Met domain, complete loss of activity
-
C483A
-
mutation in the conserved C479xxxC483xxC486 motif of the radical-Ado-Met domain, complete loss of activity
-
C486A
-
mutation in the conserved C479xxxC483xxC486 motif of the radical-Ado-Met domain, complete loss of activity
-
C486S
-
loss of activity
-
D597A
-
loss of activity
-
E532A
-
mutation in a candidate GGE motif in the radical Ado-Met domain, no loss of activity
-
E550A
-
mutation in a candidate GGE motif in the radical Ado-Met domain, complete loss of activity
-
S595A
-
loss of activity
-