4.1.3.44: tRNA 4-demethylwyosine synthase (AdoMet-dependent)
This is an abbreviated version!
For detailed information about tRNA 4-demethylwyosine synthase (AdoMet-dependent), go to the full flat file.
Word Map on EC 4.1.3.44
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4.1.3.44
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wybutosine
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decoding
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anticodon
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tricyclic
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guanosine
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codon-anticodon
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s-adenosyl-l-methionine
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5\'-deoxyadenosyl
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3'-position
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iron-sulfur
- 4.1.3.44
- wybutosine
-
decoding
-
anticodon
-
tricyclic
- guanosine
-
codon-anticodon
- s-adenosyl-l-methionine
-
5\'-deoxyadenosyl
-
3'-position
-
iron-sulfur
Reaction
Synonyms
4-demethylwyosine synthase, Taw1, tRNA wyosine derivatives biosynthesis protein Taw1, TYW1, YPL207W
ECTree
4.1.3.44 tRNA 4-demethylwyosine synthase (AdoMet-dependent)Advanced search results
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results (Crystallization
Crystallization on EC 4.1.3.44 - tRNA 4-demethylwyosine synthase (AdoMet-dependent)
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 100 mM KH2PO4/Na2HPO4 pH 6.2, 10% (w/v) PEG 3000
to 2.4 A resolution. Enzyme TYW1 assumes an incomplete TIM barrel with (alpha/beta)6 topology, which closely resembles the reported structures of radical SAM enzymes. TYW1 is considered to catalyze the cyclization reaction by utilizing the radical intermediate. construction of a model structure complexed with S-adenosylmethionine and two [4Fe-4S] clusters
to 2.2 A resolution. The overall structure is a half-barrel structure, composed of six alpha-helices packed against a ten-stranded beta-sheet. Other alpha-helices and two long loops form the walls on both sides of the half-barrel. Construction of a docking model with the FeS clusters, AdoMet and the tRNA
