4.1.3.43: 4-hydroxy-2-oxohexanoate aldolase
This is an abbreviated version!
For detailed information about 4-hydroxy-2-oxohexanoate aldolase, go to the full flat file.
Reaction
Synonyms
BphI, TTHB246
ECTree
4.1.3.43 4-hydroxy-2-oxohexanoate aldolaseAdvanced search results
results (
results (KCat KM Value
KCat KM Value on EC 4.1.3.43 - 4-hydroxy-2-oxohexanoate aldolase
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kcat/KM VALUE [1/mMs-1] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.0037
glycolaldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0005
Isobutyraldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.1
(4R)-4-hydroxy-2-oxopentanoate
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.13
(4R)-4-hydroxy-2-oxopentanoate
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
10.4
(4R)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
10.4
(4R)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
14.1
(4R)-4-hydroxy-2-oxopentanoate
-
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.012
(4S)-4-hydroxy-2-oxopentanoate
-
R16K variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.34
(4S)-4-hydroxy-2-oxopentanoate
-
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.45
(4S)-4-hydroxy-2-oxopentanoate
-
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
12.6
(4S)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
12.6
(4S)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
13.4
(4S)-4-hydroxy-2-oxopentanoate
-
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
44.7
(4S)-4-hydroxy-2-oxopentanoate
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
44.7
(4S)-4-hydroxy-2-oxopentanoate
-
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
5.2
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
10.1
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
10.6
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
13.5
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
33.3
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
0.8
4-hydroxy-2-oxopentanoate
with app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C
1.6
4-hydroxy-2-oxopentanoate
with app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C
4.9
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
7.8
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
9.3
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
11
4-hydroxy-2-oxopentanoate
with app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C
13
4-hydroxy-2-oxopentanoate
with app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C
14.6
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
19
4-hydroxy-2-oxopentanoate
with app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C
46.1
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
0.00042
acetaldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00049
acetaldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0025
acetaldehyde
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0043
acetaldehyde
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0069
acetaldehyde
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0117
acetaldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0134
acetaldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0134
acetaldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0134
acetaldehyde
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00016
Butyraldehyde
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00031
Butyraldehyde
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00079
Butyraldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00104
Butyraldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0015
Butyraldehyde
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00219
Butyraldehyde
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0022
Butyraldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0022
Butyraldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0129
Butyraldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00001
hexaldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00001
hexaldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0001
hexaldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00013
hexaldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0058
oxaloacetate
-
L87W variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.064
oxaloacetate
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.098
oxaloacetate
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.254
oxaloacetate
-
L87N variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.53
oxaloacetate
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
2.2
oxaloacetate
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00004
pentaldehyde
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.000051
pentaldehyde
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00033
pentaldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00047
pentaldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.00047
pentaldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00047
pentaldehyde
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0017
pentaldehyde
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0038
pentaldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0233
pentaldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0018
propionaldehyde
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.002
propionaldehyde
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0057
propionaldehyde
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00673
propionaldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0132
propionaldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0132
propionaldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0132
propionaldehyde
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0156
propionaldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0175
propionaldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0036
pyruvate
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0043
pyruvate
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0084
pyruvate
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0159
pyruvate
-
L87A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0587
pyruvate
-
L89A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0915
pyruvate
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
additional information
additional information
highest catalytic efficiency of BphI occurs when NADH is present, which is 25fold higher than the value obtained without nucleotides
-
additional information
additional information
-
value not detectable for R16A variant of BphI
-
