4.1.3.43: 4-hydroxy-2-oxohexanoate aldolase

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For detailed information about 4-hydroxy-2-oxohexanoate aldolase, go to the full flat file.

Reaction

Synonyms

BphI, TTHB246

ECTree

4 Lyases

4.1 Carbon-carbon lyases

4.1.3 Oxo-acid-lyases

4.1.3.43 4-hydroxy-2-oxohexanoate aldolase

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Results	in table
1	Activating Compound
379	AA Sequence
6	Cloned(Commentary)
4	General Information
11	Inhibitors
89	kcat/KM [mM/s]
7	Ki Value [mM]
90	KM Value [mM]
1	Metals/Ions
10	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
6	Organism
8	Pathway
24	Protein Variants
3	Purification (Commentary)
1	Reaction
1	Reaction Type
6	Reference
1	Storage Stability
36	Substrates and Products (Substrate)
2	Subunits
6	Synonyms
1	Systematic Name
3	Temperature Stability [°C]
89	Turnover Number [1/s]

Cloned

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Cloned on EC 4.1.3.43 - 4-hydroxy-2-oxohexanoate aldolase

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bphI and bphJ cloned into the plasmids pBTL4-T7 and pET28a

Paraburkholderia xenovorans

721651

bphI previous to mutagenesis cloned into plasmid pBTL4-T7, Escherichia coli

Paraburkholderia xenovorans

714244

chimeric complexes of Burkholderia xenovorans and Thermus thermophilus enzymes, TTHB246-BphJ and BphI-TTHB247 created by coexpression of the relevant genes in Escherichia coli using separate expression plasmids

Thermus thermophilus

Q53WI0

721659

coexpression of bphI and bphJ in Escherichia coli using two compatible plasmids (pBTL4 and pET28a) yielded soluble proteins

Paraburkholderia xenovorans

P51015

721615

separate expression of TTHB246 in recombinant Escherichia coli

Thermus thermophilus

Q53WI0

721659

variants of BphI expressed in recombinant Escherichia coli BL21, lambdaDE3 cells

Paraburkholderia xenovorans

722458