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4.1.3.42: (4S)-4-hydroxy-2-oxoglutarate aldolase

This is an abbreviated version!
For detailed information about (4S)-4-hydroxy-2-oxoglutarate aldolase, go to the full flat file.

Reaction

(4S)-4-hydroxy-2-oxoglutarate
=
pyruvate
+
glyoxylate

Synonyms

2-keto-4-hydroxybutyrate aldolase, 2-keto-4-hydroxyglutarate aldolase, 2-keto-4-hydroxyglutaric aldolase, 2-oxo-4-hydroxyglutarate aldolase, 2-oxo-4-hydroxyglutaric aldolase, 4-hydroxy-2-ketoglutarate aldolase, 4-hydroxy-2-ketoglutaric aldolase, 4-hydroxy-2-oxoglutarate glyoxylate-lyase, eda, HGA, hydroxyketoglutarate aldolase, Hydroxyketoglutaric aldolase, KHG/KDPG aldolase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.42 (4S)-4-hydroxy-2-oxoglutarate aldolase

Inhibitors

Inhibitors on EC 4.1.3.42 - (4S)-4-hydroxy-2-oxoglutarate aldolase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Ketoglutarate
-
-
4-(Oxyacetyl)phenoxyacetic acic
-
inactivates arginine residues, 90% inactivation at 10 mM
4-mercuribenzenesulfonic acid
1 mM, 56% inhibition
acetaldehyde
-
Ki: 11 mM
Bromopyruvate
85% inhibition at 1 mM, pyruvate or 2-keto-4-hydroxyglutarate protect; substrate analog. Treatment results in a time- and concentration-dependent loss of enzymatic activity. The substrates pyruvate and 2-keto-4-hydroxyglutarate provide more than 90% protection against inactivation by bromopyruvate, no protective effect is seen with glycolaldehyde. 1.1 mol of bromopyruvate is incorporated per enzyme subunit by esterification of residue Glu45
chloride
-
41% inhibition at 40 mM
citrate
-
Ki: 7.5 mM
CN-
irreversible, in presence of 4-hydroxy-2-oxoglutarate; irreversible loss of activity in presence of glyoxylate, but not in presence of pyruvate
glyoxylate
Halides
-
-
-
Hydroxypyruvate
-
Ki: 0.0125 mM
iodoacetate
46% inhibition at 5 mM; 50 mM, almost complete inhibition
N-ethylmaleimide
10% inhibition at 2.5 mM
NaBH4
extensive loss of activity after incubation of enzyme with NaBH4 in presence of pyruvate or glyoxylate
NaBr
-
48% inhibition at 20 mM
NaCl
-
38% inhibition at 20 mM
NaF
-
19% inhibition at 20 mM
NaI
-
59% inhibition at 20 mM
p-Mercuriphenylsulfonate
50% inhibition at 0.5 mM
pyruvate
-
competitive inhibition of glyoxylate binding
sulfhydryl-reacting reagents
-
partial
-
additional information
-