4.1.3.42: (4S)-4-hydroxy-2-oxoglutarate aldolase
This is an abbreviated version!
For detailed information about (4S)-4-hydroxy-2-oxoglutarate aldolase, go to the full flat file.
Reaction
Synonyms
2-keto-4-hydroxybutyrate aldolase, 2-keto-4-hydroxyglutarate aldolase, 2-keto-4-hydroxyglutaric aldolase, 2-oxo-4-hydroxyglutarate aldolase, 2-oxo-4-hydroxyglutaric aldolase, 4-hydroxy-2-ketoglutarate aldolase, 4-hydroxy-2-ketoglutaric aldolase, 4-hydroxy-2-oxoglutarate glyoxylate-lyase, eda, HGA, hydroxyketoglutarate aldolase, Hydroxyketoglutaric aldolase, KHG/KDPG aldolase
ECTree
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Inhibitors
Inhibitors on EC 4.1.3.42 - (4S)-4-hydroxy-2-oxoglutarate aldolase
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4-(Oxyacetyl)phenoxyacetic acic
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inactivates arginine residues, 90% inactivation at 10 mM
Bromopyruvate
85% inhibition at 1 mM, pyruvate or 2-keto-4-hydroxyglutarate protect; substrate analog. Treatment results in a time- and concentration-dependent loss of enzymatic activity. The substrates pyruvate and 2-keto-4-hydroxyglutarate provide more than 90% protection against inactivation by bromopyruvate, no protective effect is seen with glycolaldehyde. 1.1 mol of bromopyruvate is incorporated per enzyme subunit by esterification of residue Glu45
CN-
irreversible, in presence of 4-hydroxy-2-oxoglutarate; irreversible loss of activity in presence of glyoxylate, but not in presence of pyruvate
NaBH4
extensive loss of activity after incubation of enzyme with NaBH4 in presence of pyruvate or glyoxylate
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bovine enzyme inhibited at lower concentrations than E. coli enzyme
glyoxylate
substrate inhibition. Glyoxylate competes for, and inhibits aldolase activity by reacting with, the one active-site lysine residue/subunit
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not inhibitory: EDTA, 1,10-phenanthroline, S-hydroxyquinoline, or alpha,alpha'-dipyridyl
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additional information
not inhibitory: EDTA, 1,10-phenanthroline, S-hydroxyquinoline, or alpha,alpha'-dipyridyl
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