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4.1.3.36: 1,4-dihydroxy-2-naphthoyl-CoA synthase

This is an abbreviated version!
For detailed information about 1,4-dihydroxy-2-naphthoyl-CoA synthase, go to the full flat file.

Word Map on EC 4.1.3.36

Reaction

4-(2-carboxyphenyl)-4-oxobutanoyl-CoA
=
1,4-dihydroxy-2-naphthoyl-CoA
 +
H2O

Synonyms

1,4-dihydroxy-2-naphthoate synthetase, 1,4-dihydroxy-2-naphthoyl coenzyme A synthase, 1,4-dihydroxy-2-naphthoyl-CoA synthase, 1,4-dihydroxy-2-naphthoyl-coenzyme synthase, DHNA synthetase, DHNA-CoA synthase, dihydroxynaphthoate synthase, Dihydroxynaphthoic acid synthetase, MenB, naphthoate synthase, naphthoate synthase (MenB), Rv0548c, synthase, 1,4-dihydroxy-2-naphthoate

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.36 1,4-dihydroxy-2-naphthoyl-CoA synthase

Engineering

Engineering on EC 4.1.3.36 - 1,4-dihydroxy-2-naphthoyl-CoA synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D163A
-
loss of the ligand binding ability
D163E
-
14% 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
D163N
-
no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
F270A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
G156D
-
no activity
K89A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
K91A
-
site-directed mutagenesis, inactive mutant
R267A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R91A
-
no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
S161A
-
expressed in inclusion bodies and unavailable for the binding test
Y258F
-
no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
Y94F
-
wild type level activity