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4.1.3.32: 2,3-Dimethylmalate lyase

This is an abbreviated version!
For detailed information about 2,3-Dimethylmalate lyase, go to the full flat file.

Word Map on EC 4.1.3.32

Reaction

(2R,3S)-2,3-dimethylmalate
=
propanoate
+
pyruvate

Synonyms

(2R,3S)-dimethylmalate lyase, 2,3-dimethylmalate lyase, An07g08390, DMML, Lyase, 2,3-dimethylmalate

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.32 2,3-Dimethylmalate lyase

Purification

Purification on EC 4.1.3.32 - 2,3-Dimethylmalate lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
(NH4)2SO4 precipitation, Source Phe column chromatography, and Source 15Q FPLC column chromatography
-
by using a column chromatography-based protocol
Q-Sepharose column chromatography, ammonium sulfate precipitation, butyl Sepharose column chromatography, and phenyl Sepharose column chromatography