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4.1.3.32: 2,3-Dimethylmalate lyase

This is an abbreviated version!
For detailed information about 2,3-Dimethylmalate lyase, go to the full flat file.

Word Map on EC 4.1.3.32

Reaction

(2R,3S)-2,3-dimethylmalate
=
propanoate
+
pyruvate

Synonyms

(2R,3S)-dimethylmalate lyase, 2,3-dimethylmalate lyase, An07g08390, DMML, Lyase, 2,3-dimethylmalate

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.32 2,3-Dimethylmalate lyase

Crystallization

Crystallization on EC 4.1.3.32 - 2,3-Dimethylmalate lyase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DMML in complex with Mg2+ and in complex with Mg2+ and 3,3-difluorooxalacetate, hanging drop vapor diffusion method, using 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane (pH 7.5), and 20k polyethylene glycol 3350, or using 14k polyethylene glycol 6000 and 0.1 M MES (pH 6.5)
in complex with Mg2+, hanging drop vapour diffusion method
the crystal structure of DMML in complex with Mg2+ and in complex with Mg2+ and a substrate analog, the gem-diol of 3,3-difluoro-oxaloacetate, is determined