4.1.3.32: 2,3-Dimethylmalate lyase

This is an abbreviated version!
For detailed information about 2,3-Dimethylmalate lyase, go to the full flat file.

(2R,3S)-dimethylmalate lyase, 2,3-dimethylmalate lyase, An07g08390, DMML, Lyase, 2,3-dimethylmalate

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Results	in table
2	Activating Compound
376	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
3	Crystallization (Commentary)
1	General Information
14	Inhibitors
13	kcat/KM [mM/s]
16	Ki Value [mM]
54	KM Value [mM]
12	Metals/Ions
12	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
8	Organism
3	Pathway
28	PDB ID
3	pH Optimum
1	pH Range
20	Protein Variants
4	Purification (Commentary)
2	Reaction
8	Reference
35	Substrates and Products (Substrate)
6	Subunits
8	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
69	Turnover Number [1/s]

Cloned on EC 4.1.3.32 - 2,3-Dimethylmalate lyase

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expressed in Escherichia coli

expressed in Escherichia coli BL21 cells

expressed in Escherichia coli BL21(DE3) cells

into the vector pGEM-T Easy and subsequently into pET3c for expression in Escherichia coli BL21DE3 cells