4.1.2.8: indole-3-glycerol-phosphate lyase

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For detailed information about indole-3-glycerol-phosphate lyase, go to the full flat file.

Reaction

Synonyms

BX1, IGL, indole synthase, indole-3-glycerol phosphate lyase, indole-3-glycerophosphate D-glyceraldehyde-3-phosphate-lyase, indoleglycerolphosphate hydrolase, INS, Os03g58300, OSJNBa0094F01.14, PtIGL, TRPS, tryptophan synthase alpha, tryptophan synthase alpha subunit, TSA, Tsa1, ZmTSA

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.2 Aldehyde-lyases

                4.1.2.8 indole-3-glycerol-phosphate lyase

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1	Activating Compound
7	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
3	Crystallization (Commentary)
1	Expression
2	General Information
7	Inhibitors
1	kcat/KM [mM/s]
3	KM Value [mM]
4	Localization
8	Natural Substrates/ Products (Substrates)
15	Organism
6	Pathway
1	pH Optimum
5	Protein Variants
3	Purification (Commentary)
2	Reaction
15	Reference
12	Source Tissue
15	Substrates and Products (Substrate)
1	Subunits
23	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
3	Turnover Number [1/s]

Engineering

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Engineering on EC 4.1.2.8 - indole-3-glycerol-phosphate lyase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

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Persicaria tinctoria

A0A1C9CXC5

PtINS has about two to fivefolds higher transcript level than that of PtTSA (tryptophan synthase alpha-subunit). After treatment with 2,1,3-benzothiadiazole the relative transcript level of PtINS (indole synthase) over PtTSA (tryptophan synthase alpha-subunit) is significantly enhanced in the plant

748938

D60N

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, substitution of asparagine for aspartic acid in the alphaAsp60Asn variant. alphaAsp60 has an essential role in stabilizing charge development on the IGP indole ring during C-C bond scission

695226

Q114N

Salmonella enterica subsp. enterica serovar Typhimurium

-

betaGln114Asn mutation, which shortens the betaGln114 side chain connecting the betaL3 loop with the rest of the protein, resulted in two fractions of enzyme

691963

T183V

Salmonella enterica subsp. enterica serovar Typhimurium

-

site-directed mutagenesis, substitution of valine for threonine in the alphaThr183Val variant, resulting in impaired catalysis at the alpha-site and loss of allosteric communication

695226

Y110V

Salmonella enterica subsp. enterica serovar Typhimurium

-

betaThr110Val mutation, which replaces a hydrogen bonding partner to the substrate carboxylate with a methyl group severely decreased the ability to form the aminoacrylate external aldimine E(AA) and consequently severely impaired beta-reaction and alphabeta-reaction

691963