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0.24
DL-erythro-phenylserine
pH 8.0, 30°C
23.6
DL-threo-(3-methylsulfonylphenyl)serine
pH 7.0, 37°C
21.5
DL-threo-(3-nitrophenyl)serine
pH 7.0, 37°C
0.12 - 19.2
DL-threo-phenylserine
0.027
L-4-hydroxythreonine
-
pH 8.0, 25°C
0.052 - 31
L-allo-threonine
10.2
L-erythro-phenylserine
pH 8.0, 30°C
0.000002 - 0.0000026
L-phenylserine
0.0002 - 0.00023
L-threo-3,4-dihydroxyphenylserine
8.3
L-threo-beta-3,4-dihydroxyphenylserine
pH 8.0, 30°C
7.4
L-threo-beta-3,4-methylenedioxyphenylserine
pH 8.0, 30°C
7.3
L-threo-phenylserine
pH 8.0, 30°C
0.12
DL-threo-phenylserine
pH 8.0, 30°C
19.2
DL-threo-phenylserine
pH 7.0, 37°C
0.052
L-allo-threonine
-
pH 8.0, 25°C
0.2
L-allo-threonine
-
mutant H126NF, pH 7.0, 30°C
0.22
L-allo-threonine
pH 8.0, 30°C
0.24
L-allo-threonine
pH 7.0, 30°C, wild-type enzyme
0.24
L-allo-threonine
-
wild-type, pH 7.0, 30°C
0.31
L-allo-threonine
pH 7.0, 30°C, mutant enzyme F87A
0.41
L-allo-threonine
pH 7.0, 30°C, mutant enzyme F87D
0.69
L-allo-threonine
wild-type, pH 8.0, 30°C
0.96
L-allo-threonine
-
mutant H126N, pH 7.0, 30°C
1 - 2
L-allo-threonine
mutant D95L, pH 8.0, 30°C
1.2
L-allo-threonine
pH 7.0, 30°C, mutant enzyme K222A
1.3
L-allo-threonine
-
mutant F87A, pH 7.0, 30°C
1.5
L-allo-threonine
pH 7.0, 37°C
1.7
L-allo-threonine
-
mutant F87D, pH 7.0, 30°C
1.7
L-allo-threonine
-
mutant H83N, pH 7.0, 30°C
2.5
L-allo-threonine
mutant D95W, pH 8.0, 30°C
2.7
L-allo-threonine
mutant D95Y, pH 8.0, 30°C
2.9
L-allo-threonine
mutant D95G/E96G, pH 8.0, 30°C
4.4
L-allo-threonine
mutant D95Y/E96T, pH 8.0, 30°C
5
L-allo-threonine
-
mutant K222A, pH 7.0, 30°C
7
L-allo-threonine
-
mutant H83F, pH 7.0, 30°C
7.1
L-allo-threonine
mutant D95N/E96S, pH 8.0, 30°C
7.4
L-allo-threonine
pH 7.0, 30°C, mutant enzyme H83F/H126F
10
L-allo-threonine
pH 8.0, 30°C
11
L-allo-threonine
mutant D95C, pH 8.0, 30°C
14.6
L-allo-threonine
pH 8.0, 30°C
31
L-allo-threonine
-
mutant H83F/H126F, pH 7.0, 30°C
0.000002
L-phenylserine
-
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0)
0.0000026
L-phenylserine
-
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0)
0.00016
L-Thr
-
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0)
0.00018
L-Thr
-
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0)
0.0002
L-threo-3,4-dihydroxyphenylserine
-
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0)
0.00023
L-threo-3,4-dihydroxyphenylserine
-
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0)
0.4
L-threonine
-
mutant F87A, pH 7.0, 30°C
1 - 3
L-threonine
mutant D95G/E96G, pH 8.0, 30°C
1.1
L-threonine
-
mutant H83F/H126F, pH 7.0, 30°C
1.3
L-threonine
-
mutant F87D, pH 7.0, 30°C
1.7
L-threonine
-
mutant H126F, pH 7.0, 30°C
2.85
L-threonine
pH 8.0, 30°C
3.7
L-threonine
-
mutant K222A, pH 7.0, 30°C
4
L-threonine
-
pH 8.0, 25°C
7
L-threonine
-
mutant H83F, pH 7.0, 30°C
8.7
L-threonine
pH 7.0, 30°C, mutant enzyme F87A
13.6
L-threonine
wild-type, pH 8.0, 30°C
14.7
L-threonine
pH 8.0, 30°C
19.4
L-threonine
pH 7.0, 30°C, wild-type enzyme
19.4
L-threonine
-
wild-type, pH 7.0, 30°C
21
L-threonine
pH 7.0, 30°C, mutant enzyme H83F/H126F
24.9
L-threonine
pH 7.0, 30°C, mutant enzyme F87D
32
L-threonine
mutant D95N/E96S, pH 8.0, 30°C
38
L-threonine
-
mutant H83N, pH 7.0, 30°C
43.1
L-threonine
pH 7.0, 37°C
44
L-threonine
mutant D95C, pH 8.0, 30°C
44
L-threonine
mutant D95W, pH 8.0, 30°C
50
L-threonine
mutant D95M, pH 8.0, 30°C
53
L-threonine
mutant D95L, pH 8.0, 30°C
55
L-threonine
pH 8.0, 30°C
61
L-threonine
-
mutant H126N, pH 7.0, 30°C
63
L-threonine
mutant D95Y, pH 8.0, 30°C
72
L-threonine
pH 7.0, 30°C, mutant enzyme K222A
4.1.2.48 low-specificity L-threonine aldolase
results (
results (
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