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4.1.2.46: aliphatic (R)-hydroxynitrile lyase

This is an abbreviated version!
For detailed information about aliphatic (R)-hydroxynitrile lyase, go to the full flat file.

Reaction

(2R)-2-hydroxy-2-methylbutanenitrile
=
cyanide
+
butan-2-one

Synonyms

(R)-HNL, (R)-hydroxynitrile lyase, (R)-Oxynitrilase, Acetone cyanohydrin lyase, EC 4.1.2.37, Hydroxynitrile lyase, LuHNL

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.2 Aldehyde-lyases
                4.1.2.46 aliphatic (R)-hydroxynitrile lyase

Cloned

Cloned on EC 4.1.2.46 - aliphatic (R)-hydroxynitrile lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into Pichia pastoris, expressed in Escherichia coli as an N-terminal hexa-histidine fusion protein
-
cloning of a myc-His-tagged LuHNL-cDNA under control of the methanol-inducible AOX1 (alcohol oxidase) promotor of Pichia pastoris and introduction in the SMD1168 strain. Recombinant LuHNL is kinetically indistinguishable from the authentic flax enzyme
expressed in Escherichia coli as N-terminal hexa-histidine fusion protein
expression in Escherichia coli