4.1.2.46: aliphatic (R)-hydroxynitrile lyase
This is an abbreviated version!
For detailed information about aliphatic (R)-hydroxynitrile lyase, go to the full flat file.
Reaction
Synonyms
(R)-HNL, (R)-hydroxynitrile lyase, (R)-Oxynitrilase, Acetone cyanohydrin lyase, EC 4.1.2.37, Hydroxynitrile lyase, LuHNL
ECTree
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Cloned
Cloned on EC 4.1.2.46 - aliphatic (R)-hydroxynitrile lyase
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cloned into Pichia pastoris, expressed in Escherichia coli as an N-terminal hexa-histidine fusion protein
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cloning of a myc-His-tagged LuHNL-cDNA under control of the methanol-inducible AOX1 (alcohol oxidase) promotor of Pichia pastoris and introduction in the SMD1168 strain. Recombinant LuHNL is kinetically indistinguishable from the authentic flax enzyme
expressed in Escherichia coli as N-terminal hexa-histidine fusion protein